Cloned (Comment) | Organism |
---|---|
liver enzyme | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R196G | decrease in kcat/Km | Rattus norvegicus |
S249K | decrease in kcat/Km | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? | |
additional information | Rattus norvegicus | cyanide detoxification | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin | mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
cerebrum | - |
Rattus norvegicus | - |
glial cell | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | predominantly in tubular epithelium | Rattus norvegicus | - |
liver | predominantly in pericentral hepatocytes | Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? | |
additional information | cyanide detoxification | Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0783 | - |
3-Mercaptopyruvate | S249K mutant | Rattus norvegicus | |
0.233 | - |
3-Mercaptopyruvate | G248R mutant | Rattus norvegicus | |
0.917 | - |
3-Mercaptopyruvate | S249A mutant | Rattus norvegicus | |
1.03 | - |
3-Mercaptopyruvate | wild-type enzyme | Rattus norvegicus | |
1.07 | - |
3-Mercaptopyruvate | R196G mutant | Rattus norvegicus | |
5.67 | - |
3-Mercaptopyruvate | R187G mutant | Rattus norvegicus |