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Literature summary for 2.7.9.4 extracted from
- A novel type carbohydrate-binding module identified in alpha-glucan, water dikinases is specific for regulated plastidial starch metabolism (2006), Biochemistry, 45, 4674-4682.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant production of enzyme fragments SBD and N11 corresponding to the fragments resulting from limited proteolysis of enzyme by trypsin. Fragment N11 shows starch phosphorylating activity and a specific activity 2fold higher than that of wild-type and a doubling of Km value for potato starch substrate. N11 exhibits a higher preference for phosphorylating shorter chains compared to wild-type. Using amylose as substrate, N11 shows 5fold higher specific activity | Solanum tuberosum |
| W117A | mutation in tandem repeat sequence SBD-1, mutant shows extremely weak glucan binding | Solanum tuberosum |
| W62A | mutation in tandem repeat sequence SBD-1, mutant shows extremely weak glucan binding | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | Q9AWA5 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
30°C, wild-type, substrate potato amylose | Solanum tuberosum |
| 0.14 | - |
30°C, fragment N11, substrate potato amylose | Solanum tuberosum |
| 0.2 | - |
30°C, wild-type, substrate amylose 53 | Solanum tuberosum |
| 0.41 | - |
30°C, wild-type, substrate amylose 85 | Solanum tuberosum |
| 1.01 | - |
30°C, fragment N11, substrate amylose 53 | Solanum tuberosum |
| 1.18 | - |
30°C, fragment N11, substrate amylose 85 | Solanum tuberosum |
| 3.26 | - |
30°C, wild-type, substrate potato amylopektin | Solanum tuberosum |
| 5.8 | - |
30°C, wild-type, substrate granular potato starch | Solanum tuberosum |
| 6.19 | - |
30°C, fragment N11, substrate potato amylopektin | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + amylose 18 + H2O | - |
Solanum tuberosum | AMP + phosphorylated amylose 18 + phosphate | very poor substrate | ? |  |
| ATP + amylose 24 + H2O | - |
Solanum tuberosum | AMP + phosphorylated amylose 24 + phosphate | very poor substrate | ? | |
| ATP + amylose 53 + H2O | - |
Solanum tuberosum | AMP + phosphorylated amylose 53 + phosphate | - |
? | |
| ATP + amylose 85 + H2O | - |
Solanum tuberosum | AMP + phosphorylated amylose 85 + phosphate | - |
? | |
| ATP + granular potato starch + H2O | - |
Solanum tuberosum | AMP + phosphorylated granular potato starch + phosphate | - |
? | |
| ATP + potato amylopektin + H2O | - |
Solanum tuberosum | AMP + phosphorylated potato amylopektin + phosphate | - |
? | |
| ATP + potato amylose + H2O | - |
Solanum tuberosum | AMP + phosphorylated potato amylose + phosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
granular potato starch | 30°C | Solanum tuberosum |
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Release 2023.1 (January 2023)
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