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Literature summary for 2.7.8.43 extracted from
- Extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system (2015), Mol. Microbiol., 97, 166-178.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene eptA, quantitative and semi-quantitative reverse-transcriptase (RT) PCR enzyme expression analysis | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | lipid A prepared from the PA14DELTAeptAPa mutant shows no phosphatidylethanolamine modification when Zn2+ is added to the media | Pseudomonas aeruginosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system | Pseudomonas aeruginosa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylphosphatidylethanolamine + lipid A | Pseudomonas aeruginosa | - |
diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | A0A069Q704 | gene eptA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylphosphatidylethanolamine + lipid A | - |
Pseudomonas aeruginosa | diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate) | - |
? | |
| diacylphosphatidylethanolamine + lipid A | Pseudomonas aeruginosa lipid A, enzyme EptAPa-dependent addition of pEtN to the 4' phosphate group of BN2 and PA14 lipid A | Pseudomonas aeruginosa | diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EptAPa | - |
Pseudomonas aeruginosa |
| pEtN transferase | - |
Pseudomonas aeruginosa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas aeruginosa | transcription of the enzyme eptAPa is regulated by zinc via the ColRS two-component system. The two-component system response regulator ColR activates eptAPa transcription. Transcription of eptAPa is induced by 2 mM ZnSO4 approximately 21fold | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deletion of colR and of eptA results in loss of Zn2+-induced phosphatidylethanolamine modification of Pseudomonas aeruginosa lipid A. colR deletion mutant complementation restores Zn2+-dependent eptAPa transcription by more than fourfold | Pseudomonas aeruginosa |
| physiological function | Gram-negative bacteria survive harmful environmental stressors by modifying their outer membrane. Much of this protection is afforded upon remodeling of the lipid A region of the major surface molecule lipopolysaccharide. Addition of cationic substituents, such as 4-amino-4-deoxy-L-arabinose (L-Ara4N) and phosphoethanolamine (pEtN) at the lipid A phosphate groups, is often induced in response to specific environmental flux stabilizing the outer membrane. ColR specifically induces pEtN addition to lipid A in lieu of L-Ara4N when Zn2+ is present | Pseudomonas aeruginosa |
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