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Literature summary for 2.7.8.27 extracted from
- Both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells (2007), J. Biol. Chem., 282, 17537-17547.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression analysis of isozymes SMS1 and SMS2 by RT-PCR | Mus musculus |
| expression analysis of isozymes SMS1 and SMS2 by RT-PCR, recombinant overexpression of isozymes SMS1 and SMS2 in HeLa cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | endogenous SMS2 expression in HeLa cells is reduced by about 80% after treatment with SMS2 siRNA, but is unaffected after treatment with lamin A or SMS1 siRNAs. Impact of SMS1 and SMS2 depletion on the lipid composition of HeLa cells, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Mus musculus | 139 | - |
| Golgi membrane | isozyme SMS1 | Homo sapiens | 139 | - |
| additional information | immunohistochemical localization study | Homo sapiens | - |
- |
| perinuclear space | a small portion of SMS2 is found in the perinuclear region | Homo sapiens | - |
- |
| plasma membrane | - |
Mus musculus | 5886 | - |
| plasma membrane | isozyme SMS2 | Homo sapiens | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
| Mn2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells, sphingomyelin synthesis plays a critical role in cell growth and survival, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozymes SMS1 and SMS2 | - |
| Mus musculus | - |
isozymes SMS1 and SMS2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Caco-2 cell | isozymes SMS1 and SMS2 | Homo sapiens | - |
| fibroblast | isozymes SMS1 and SMS2 | Mus musculus | - |
| HeLa cell | SMS1 represents a major whereas SMS2 represents a minor SM-synthase activity in HeLa cells | Homo sapiens | - |
| Hep-G2 cell | isozymes SMS1 and SMS2 | Homo sapiens | - |
| MEB-4 cell | isozymes SMS1 and SMS2 | Mus musculus | - |
| melanoma cell | isozymes SMS1 and SMS2 | Mus musculus | - |
| myofibroblast cell line | isozymes SMS1 and SMS2 | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells, sphingomyelin synthesis plays a critical role in cell growth and survival, overview | Homo sapiens | ? | - |
? | |
| phosphatidylcholine + C6-NBD-ceramide | i.e. N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine, the fluorogenic derivative of C6-ceramide | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SM synthase | - |
Mus musculus |
| SM synthase | - |
Homo sapiens |
| SMS1 | - |
Mus musculus |
| SMS1 | - |
Homo sapiens |
| SMS2 | - |
Mus musculus |
| SMS2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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