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Literature summary for 2.7.7.9 extracted from

  • Cotrim, C.A.; Soares, J.S.M.; Kobe, B.; Menossi, M.
    Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane (2018), PLoS ONE, 13, e0193667 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Saccharum hybrid cultivar SP80-3280

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method at 20°C, crystal structure at resolution of 2.0 A. The crystals reveals the presence of two molecules in the asymmetric unit Saccharum hybrid cultivar SP80-3280

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate Saccharum hybrid cultivar SP80-3280
-
diphosphate + UDP-glucose
-
?

Organism

Organism UniProt Comment Textmining
Saccharum hybrid cultivar SP80-3280 A0A075E2Q1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharum hybrid cultivar SP80-3280

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
Saccharum hybrid cultivar SP80-3280 diphosphate + UDP-glucose
-
?

Subunits

Subunits Comment Organism
monomer multi-angle light scattering analysis shows that the enzyme forms a mixture of species ranging from monomers to larger oligomers in solution Saccharum hybrid cultivar SP80-3280
octamer multi-angle light scattering results indicate the presence of a possible octamer in solution, which might be formed by four dimers through end-to-end arrangements of two monomers similar to yeast and human proteins Saccharum hybrid cultivar SP80-3280
oligomer multi-angle light scattering analysis shows that the enzyme forms a mixture of species ranging from monomers to larger oligomers in solution Saccharum hybrid cultivar SP80-3280

Synonyms

Synonyms Comment Organism
KF278717
-
Saccharum hybrid cultivar SP80-3280
ScUGPase-1
-
Saccharum hybrid cultivar SP80-3280
UDP-glucose pyrophosphorylase
-
Saccharum hybrid cultivar SP80-3280
UGPase
-
Saccharum hybrid cultivar SP80-3280

General Information

General Information Comment Organism
metabolism in sugarcane, UDP-glucose (the product of the reaction) is a branch-point in the carbon channelling into other carbohydrates, such as sucrose and cellulose, which are the major factors for sugarcane productivity Saccharum hybrid cultivar SP80-3280