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Literature summary for 2.7.7.9 extracted from

  • Honda, Y.; Zang, Q.; Shimizu, Y.; Dadashipour, M.; Zhang, Z.; Kawarabayasi, Y.
    Increasing the thermostable sugar-1-phosphate nucleotidylyltransferase activities of the archaeal ST0452 protein through site saturation mutagenesis of the 97th amino acid position (2017), Appl. Environ. Microbiol., 83, e02291-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Sulfurisphaera tokodaii
expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
Y97A activity is 2.3fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97A the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97C activity is 1.87fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97D activity is 6.2fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97D the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97E inactive Sulfurisphaera tokodaii
Y97F activity is 1.2fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97F the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97G activity is 4.2fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97G the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97H activity is 1.6fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97H the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97I inactive Sulfurisphaera tokodaii
Y97I the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97K activity is 3.7fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97K the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97L activity is 2.4fold higher than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97L the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97M activity is 2.8fold higher than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97M the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97N activity is 4.1fold higher than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97N the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97P inactive Sulfurisphaera tokodaii
Y97Q activity is 1.04fold higher than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97Q the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97R inactive Sulfurisphaera tokodaii
Y97S activity is 1.1fold higher than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97S the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97T activity is 3.96fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97T the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97V activity is 3.34fold lower than that of the wild-type enzyme Sulfurisphaera tokodaii
Y97V the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme Sulfurisphaera tokodaii
Y97W inactive Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23
-
 alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
1.23
-
 alpha-D-glucose 1-phosphate wild type enzyme, at pH 7.5 and 80°C Sulfurisphaera tokodaii
1.74
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
1.74
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, at pH 7.5 and 80°C Sulfurisphaera tokodaii
2.5 3 alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii
2.5 3 alpha-D-glucose 1-phosphate mutant enzyme Y97N, at pH 7.5 and 80°C Sulfurisphaera tokodaii
3.28
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
3.28
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, at pH 7.5 and 80°C Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP + alpha-D-glucose 1-phosphate Sulfurisphaera tokodaii
-
 phosphate + UDP-glucose
-
 ?
UDP + alpha-D-glucose 1-phosphate Sulfurisphaera tokodaii DSM 16993
-
 phosphate + UDP-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii
His SpinTrap Talon column chromatography Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.66
-
 mutant enzyme Y97D, pH 7.5, 80°C Sulfurisphaera tokodaii
0.98
-
 mutant enzyme Y97G, pH 7.5, 80°C Sulfurisphaera tokodaii
1.03
-
 mutant enzyme Y97T, pH 7.5, 80°C Sulfurisphaera tokodaii
1.09
-
 mutant enzyme Y97K, pH 7.5, 80°C Sulfurisphaera tokodaii
1.22
-
 mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
1.74
-
 mutant enzyme Y97A, pH 7.5, 80°C Sulfurisphaera tokodaii
2.18
-
 mutant enzyme Y97C, pH 7.5, 80°C Sulfurisphaera tokodaii
3.35
-
 mutant enzyme Y97F, pH 7.5, 80°C Sulfurisphaera tokodaii
4.08
-
 wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
4.26
-
 mutant enzyme Y97Q, pH 7.5, 80°C Sulfurisphaera tokodaii
4.51
-
 mutant enzyme Y97S, pH 7.5, 80°C Sulfurisphaera tokodaii
9.97
-
 mutant enzyme Y97L, pH 7.5, 80°C Sulfurisphaera tokodaii
10.41
-
 mutant enzyme Y97H, pH 7.5, 80°C Sulfurisphaera tokodaii
11.45
-
 mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
16.92
-
 mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii phosphate + UDP-glucose
-
 ?
UDP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 phosphate + UDP-glucose
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + UDP-glucose
-
-
UTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-glucose
-
-

Synonyms

Synonyms Comment Organism
Glc-1-P UTase
-
 Sulfurisphaera tokodaii
GlcNAc-1-P UTase
-
 Sulfurisphaera tokodaii
ST0452 bifunctional enzyme exhibiting sugar-1-phosphate nucleotidylyltransferase and amino-sugar-1-phosphate acetyltransferase activities Sulfurisphaera tokodaii
ST0452 protein
-
 Sulfurisphaera tokodaii
sugar-1-P NTase
-
 Sulfurisphaera tokodaii
sugar-1-phosphate nucleotidylyltransferase
-
 Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.08
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
2.08
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, at pH 7.5 and 80°C Sulfurisphaera tokodaii
2.97
-
 alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
2.97
-
 alpha-D-glucose 1-phosphate wild type enzyme, at pH 7.5 and 80°C Sulfurisphaera tokodaii
14.97
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
14.97
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, at pH 7.5 and 80°C Sulfurisphaera tokodaii
38.15
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii
38.15
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97N, at pH 7.5 and 80°C Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.63
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C Sulfurisphaera tokodaii
0.63
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97V, at pH 7.5 and 80°C Sulfurisphaera tokodaii
2.41
-
 alpha-D-glucose 1-phosphate wild-type enzyme, pH 7.5, 80°C Sulfurisphaera tokodaii
2.41
-
 alpha-D-glucose 1-phosphate wild type enzyme, at pH 7.5 and 80°C Sulfurisphaera tokodaii
8.6
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C Sulfurisphaera tokodaii
8.6
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97M, at pH 7.5 and 80°C Sulfurisphaera tokodaii
15.08
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C Sulfurisphaera tokodaii
15.08
-
 alpha-D-glucose 1-phosphate mutant enzyme Y97N, at pH 7.5 and 80°C Sulfurisphaera tokodaii