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Literature summary for 2.7.7.88 extracted from

  • Ogino, T.; Banerjee, A.K.
    Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus (2007), Mol. Cell, 25, 85-97.
    View publication on PubMed
Search for more literature for 2.7.7.88

Inhibitors

Inhibitors Comment Organism Structure
diphosphate inhibits formation of the enzyme-RNa complex vesicular stomatitis Indiana virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ assay in presence of 1 mM divalent cation, Mn2+ is preferred over Mg2+ vesicular stomatitis Indiana virus
Mn2+ assay in presence of 1 mM divalent cation, Mn2+ is preferred over Mg2+ vesicular stomatitis Indiana virus

Organism

Organism UniProt Comment Textmining
vesicular stomatitis Indiana virus P03523
-
-
vesicular stomatitis Indiana virus San Juan P03523
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-triphospho-mRNA + GDP
-
 vesicular stomatitis Indiana virus diphosphate + guanosine 5'-triphospho-mRNA
-
 ?
5'-triphospho-mRNA + GDP
-
 vesicular stomatitis Indiana virus San Juan diphosphate + guanosine 5'-triphospho-mRNA
-
 ?
additional information a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate. The enzyme uses only 5'-triphosphorylated RNA substrates. It efficiently caps RNAs containing an A(purine)CNG sequence with GDP. Especially, the first A and third pyrimidine residues appear to be essential for the RNA substrate activity. No substrates: ppApApCpApG or pppApCpGpApA vesicular stomatitis Indiana virus ?
-
 ?
additional information a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate. The enzyme uses only 5'-triphosphorylated RNA substrates. It efficiently caps RNAs containing an A(purine)CNG sequence with GDP. Especially, the first A and third pyrimidine residues appear to be essential for the RNA substrate activity. No substrates: ppApApCpApG or pppApCpGpApA vesicular stomatitis Indiana virus San Juan ?
-
 ?
pppApApCpApG + GDP
-
 vesicular stomatitis Indiana virus diphosphate + guanosine 5'-pppApApCpApG recombinant enzyme specifically caps pppApApCpApG with GDP ?
pppApApCpApG + GDP
-
 vesicular stomatitis Indiana virus San Juan diphosphate + guanosine 5'-pppApApCpApG recombinant enzyme specifically caps pppApApCpApG with GDP ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at vesicular stomatitis Indiana virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at vesicular stomatitis Indiana virus