Literature summary for 2.7.7.73 extracted from

Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1) (2003), Biochemistry, 42, 12430-12438.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O31619	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [sulfur-carrier protein ThiS]	-	Bacillus subtilis	diphosphate + adenylyl-[sulfur-carrier protein ThiS]	-	?

Synonyms

Synonyms	Comment	Organism
ThiF	-	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Bacillus subtilis

General Information

General Information	Comment	Organism
metabolism	ThiF participates in the biosynthesis of thiazole phosphate	Bacillus subtilis

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Release 2023.1 (January 2023)