Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)
Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.; Biochemistry 42, 12430-12438 (2003) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Bacillus subtilis
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O31619
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + [sulfur-carrier protein ThiS]
-
707466
Bacillus subtilis
diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ThiF
-
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + [sulfur-carrier protein ThiS]
-
707466
Bacillus subtilis
diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
ThiF participates in the biosynthesis of thiazole phosphate
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
ThiF participates in the biosynthesis of thiazole phosphate
Bacillus subtilis
Other publictions for EC 2.7.7.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721602
Lehmann
Structure of the Escherichia c ...
Escherichia coli
Biochemistry
45
11-19
2006
-
-
1
1
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1
1
-
-
1
-
-
1
-
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1
1
1
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1
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1
1
1
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1
1
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1
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1
1
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722948
Duda
Structural analysis of Escheri ...
Escherichia coli
J. Mol. Biol.
349
774-786
2005
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1
1
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2
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1
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1
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2
1
1
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1
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1
1
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2
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1
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2
1
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1
1
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707466
Park
Biosynthesis of the thiazole m ...
Bacillus subtilis
Biochemistry
42
12430-12438
2003
-
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1
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1
-
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-
1
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1
1
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723670
Xi
Biosynthesis of the thiazole m ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
98
8513-8518
2001
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1
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1
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722608
Taylor
Thiamin biosynthesis in Escher ...
Escherichia coli
J. Biol. Chem.
273
16555-16560
1998
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