Information on EC 2.7.7.73 - sulfur carrier protein ThiS adenylyltransferase

for references in articles please use BRENDA:EC2.7.7.73
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.73
-
RECOMMENDED NAME
GeneOntology No.
sulfur carrier protein ThiS adenylyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [ThiS] = diphosphate + adenylyl-[ThiS]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
thiazole biosynthesis I (facultative anaerobic bacteria)
-
-
thiazole biosynthesis II (aerobic bacteria)
-
-
vitamin B1 metabolism
-
-
Thiamine metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:[ThiS] adenylyltransferase
Binds Zn2+. The enzyme catalyses the adenylation of ThiS, a sulfur carrier protein involved in the biosynthesis of thiamine. The enzyme shows significant structural similarity to ubiquitin-activating enzyme [3,4]. In Escherichia coli, but not in Bacillus subtilis, the enzyme forms a cross link from Cys-184 to the ThiS carboxy terminus (the position that is also thiolated) via an acyldisulfide [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + [ThiS]
diphosphate + adenylyl-COAMP
show the reaction diagram
-
-
-
-
?
ATP + [ThiS]
diphosphate + adenylyl-[ThiS]
show the reaction diagram
additional information
?
-
-
ThiF shows no activity with GTP, CTP or TTP as cosubstrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [ThiS]
diphosphate + adenylyl-COAMP
show the reaction diagram
-
-
-
-
?
ATP + [ThiS]
diphosphate + adenylyl-[ThiS]
show the reaction diagram
P30138
this reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
2 * 27000, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with ThiS, hanging drop vapor diffusion method, using 7-8% polyethylene glycol 400, 35 mM calcium chloride, and 100 mM Tris-HCl (pH 7.0-7.3)
-
ThiF alone and in complex with ATP, hanging drop vapor diffusion method, using 14% (w/v) ammonium acetate, 50 mM MES (pH 6.5), 5 mM dithiothreitol, at 25°C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE column chromatography, and Superdex 75 gel filtration
-
G-75 Sephadex gel filtration
-
glutathione affinity column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
-
Hi-Trap QFF column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL834(DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C184S
-
inactive, C184S is equally effective in catalyzing the formation of [ThiS]-COSH as the wild type enzyme. Cys184 is directly involved in the crosslinking of ThiF to ThiS but is not essential for the formation of [ThiS]-COSH
Show AA Sequence (472 entries)
Please use the Sequence Search for a specific query.