BRENDA - Enzyme Database
show all sequences of 2.7.7.53

Roles of Ala-149 in the catalytic activity of diadenosine tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

Mori, S.; Kim, H.; Rimbara, E.; Arakawa, Y.; Shibayama, K.; Biosci. Biotechnol. Biochem. 79, 236-238 (2015)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A149Del
an Ala149 deletion mutant displays substrate specificity for diadenosine 5',5'''-P1,P5-pentaphosphate and is inactive on Ap4A and other substrates utilized by the wild-type enzyme
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WMK9
-
-
Mycobacterium tuberculosis H37Rv
P9WMK9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
wild-type, 19% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 29%, respectively
737871
Mycobacterium tuberculosis
2 ADP
-
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + phosphate
-
737871
Mycobacterium tuberculosis
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + phosphate
-
737871
Mycobacterium tuberculosis H37Rv
ADP + ATP
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
wild-type, 107% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 53%, respectively
737871
Mycobacterium tuberculosis
GTP + GDP
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
wild-type, 107% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 53%, respectively
737871
Mycobacterium tuberculosis H37Rv
GTP + GDP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 290%, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 290%, respectively
737871
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
P1,P5-bis(5'-guanosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 95%, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + phosphate
wild-type, 25% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, very poor substrate for mutant A149Del, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + phosphate
wild-type, 25% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, very poor substrate for mutant A149Del, respectively
737871
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
wild-type, 100% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 67%, respectively
737871
Mycobacterium tuberculosis
ATP + GDP
-
-
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
wild-type, 100% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 67%, respectively
737871
Mycobacterium tuberculosis H37Rv
ATP + GDP
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A149Del
an Ala149 deletion mutant displays substrate specificity for diadenosine 5',5'''-P1,P5-pentaphosphate and is inactive on Ap4A and other substrates utilized by the wild-type enzyme
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
wild-type, 19% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 29%, respectively
737871
Mycobacterium tuberculosis
2 ADP
-
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + phosphate
-
737871
Mycobacterium tuberculosis
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + phosphate
-
737871
Mycobacterium tuberculosis H37Rv
ADP + ATP
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
wild-type, 107% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 53%, respectively
737871
Mycobacterium tuberculosis
GTP + GDP
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
wild-type, 107% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 53%, respectively
737871
Mycobacterium tuberculosis H37Rv
GTP + GDP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 290%, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 290%, respectively
737871
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
P1,P5-bis(5'-guanosyl)pentaphosphate + phosphate
wild-type, 106% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 95%, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + phosphate
wild-type, 25% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, very poor substrate for mutant A149Del, respectively
737871
Mycobacterium tuberculosis
?
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + phosphate
wild-type, 25% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, very poor substrate for mutant A149Del, respectively
737871
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
wild-type, 100% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 67%, respectively
737871
Mycobacterium tuberculosis
ATP + GDP
-
-
-
?
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
wild-type, 100% of the rate with P1,P4-bis(5'-adenosyl) tetraphosphate, mutant A149Del 67%, respectively
737871
Mycobacterium tuberculosis H37Rv
ATP + GDP
-
-
-
?
Other publictions for EC 2.7.7.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737871
Mori
Roles of Ala-149 in the cataly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biosci. Biotechnol. Biochem.
79
236-238
2015
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739577
Honda
Purification and functional ch ...
Mycobacterium avium 104, Mycobacterium avium, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Protein Expr. Purif.
112
37-42
2015
-
-
2
-
-
-
-
-
-
8
2
-
-
7
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722980
Mori
Structural insights into the n ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
410
93-104
2011
-
-
1
1
8
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
713455
Mori
Purification and molecular cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
69
99-105
2010
-
-
1
-
-
-
-
2
-
4
2
-
-
6
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
2
-
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
643515
McLennan
Anabaena flos-aquae and other ...
Dolichospermum flos-aquae, Synechococcus sp., Trichormus variabilis
Biochem. J.
320
795-800
1996
-
-
-
-
-
-
-
1
-
5
3
-
-
10
-
-
1
-
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
3
-
-
-
-
1
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Acanthamoeba castellanii
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643514
Booth
An alleged yeast polyphosphate ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
19377-19382
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209909
McLennan
The green alga Scenedesmus obl ...
Chlorella vulgaris, Scenedesmus basiliensis, Scenedesmus quadricauda, Tetradesmus obliquus
Biochem. J.
300
183-189
1994
-
-
-
-
-
-
1
7
-
7
2
-
-
9
-
-
1
-
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
7
2
-
-
-
-
1
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
643509
Avila
Immunoaffinity chromatography ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CGY 339
Biotechnol. Appl. Biochem.
12
276-283
1990
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
643513
Guranowski
Specific phosphorylase from Eu ...
Acanthamoeba castellanii, Euglena gracilis
Int. J. Biochem.
20
449-455
1988
-
-
-
-
-
-
-
3
-
2
1
-
-
2
-
-
1
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Saccharomyces cerevisiae
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643508
Brevet
Yeast diadenosine 5,5-P1,P4-te ...
Saccharomyces cerevisiae
Biochemistry
26
4763-4768
1987
-
-
-
-
-
-
1
2
-
6
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
6
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643510
Guranowski
Diadenosine 5,5-P1, P4-tetraph ...
Saccharomyces cerevisiae
J. Biol. Chem.
261
5943-5946
1986
-
-
-
-
-
2
8
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
4
-
2
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
643507
Guranowski
Phosphorolytic cleavage of dia ...
Saccharomyces cerevisiae
J. Biol. Chem.
260
3542-3547
1985
-
-
-
-
-
1
4
3
-
8
1
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
8
1
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-