BRENDA - Enzyme Database
show all sequences of 2.7.7.53

Yeast diadenosine 5,5-P1,P4-tetraphosphate alpha,beta-phosphorylase behaves as a dinucleoside tetraphosphate synthetase

Brevet, A.; Coste, H.; Fromant, M.; Plateau, P.; Blanquet, S.; Biochemistry 26, 4763-4768 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
ADP
pH 5.9, 37°C
Saccharomyces cerevisiae
5.7
-
ATP
pH 5.9, 37°C
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
as good as Mn2+
Saccharomyces cerevisiae
Co2+
slight activation of P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
Mg2+
activation, P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis; less effective than Mn2+ or Ca2+
Saccharomyces cerevisiae
Mn2+
requirement, Ap4A-synthesis
Saccharomyces cerevisiae
additional information
Cu2+; no activation by Ni2+
Saccharomyces cerevisiae
Zn2+
slight activation of P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
Saccharomyces cerevisiae
involved in catabolism of dinucleoside polyphosphates
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + ATP
-
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
r
NTP + N'DP
asymmetric catalytic site: synthesis of Ap4G or Ap4C is much faster from ATP plus CDP or GDP than from CTP or GTP plus ADP
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
N is A or G, N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
?
NTP + N'DP
N is A or G, not C, U or dA, and N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
N is A or G, N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
?
P1(5'-adenosyl)-P4(5'-cytosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
CDP + ATP
not CTP + ADP
643508
Saccharomyces cerevisiae
?
P1(5'-adenosyl)-P4(5'-guanosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
GDP + ATP
-
643508
Saccharomyces cerevisiae
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
ADP + ATP
-
643508
Saccharomyces cerevisiae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
involved in catabolism of dinucleoside polyphosphates
643508
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
8
-
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
ADP
pH 5.9, 37°C
Saccharomyces cerevisiae
5.7
-
ATP
pH 5.9, 37°C
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
as good as Mn2+
Saccharomyces cerevisiae
Co2+
slight activation of P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
Mg2+
activation, P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis; less effective than Mn2+ or Ca2+
Saccharomyces cerevisiae
Mn2+
requirement, Ap4A-synthesis
Saccharomyces cerevisiae
additional information
Cu2+; no activation by Ni2+
Saccharomyces cerevisiae
Zn2+
slight activation of P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
Saccharomyces cerevisiae
involved in catabolism of dinucleoside polyphosphates
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + ATP
-
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
r
NTP + N'DP
asymmetric catalytic site: synthesis of Ap4G or Ap4C is much faster from ATP plus CDP or GDP than from CTP or GTP plus ADP
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
N is A or G, N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
?
NTP + N'DP
N is A or G, not C, U or dA, and N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
P1,P4-bis(5'-nucleosyl) tetraphosphate' + phosphate
N is A or G, N' is A, C, G, U or dA
643508
Saccharomyces cerevisiae
?
P1(5'-adenosyl)-P4(5'-cytosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
CDP + ATP
not CTP + ADP
643508
Saccharomyces cerevisiae
?
P1(5'-adenosyl)-P4(5'-guanosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
GDP + ATP
-
643508
Saccharomyces cerevisiae
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643508
Saccharomyces cerevisiae
ADP + ATP
-
643508
Saccharomyces cerevisiae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
involved in catabolism of dinucleoside polyphosphates
643508
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis
Saccharomyces cerevisiae
8
-
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae
Other publictions for EC 2.7.7.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737871
Mori
Roles of Ala-149 in the cataly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biosci. Biotechnol. Biochem.
79
236-238
2015
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739577
Honda
Purification and functional ch ...
Mycobacterium avium 104, Mycobacterium avium, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Protein Expr. Purif.
112
37-42
2015
-
-
2
-
-
-
-
-
-
8
2
-
-
7
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722980
Mori
Structural insights into the n ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
410
93-104
2011
-
-
1
1
8
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
713455
Mori
Purification and molecular cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
69
99-105
2010
-
-
1
-
-
-
-
2
-
4
2
-
-
6
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
2
-
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
643515
McLennan
Anabaena flos-aquae and other ...
Dolichospermum flos-aquae, Synechococcus sp., Trichormus variabilis
Biochem. J.
320
795-800
1996
-
-
-
-
-
-
-
1
-
5
3
-
-
10
-
-
1
-
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
3
-
-
-
-
1
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Acanthamoeba castellanii
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643514
Booth
An alleged yeast polyphosphate ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
19377-19382
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209909
McLennan
The green alga Scenedesmus obl ...
Chlorella vulgaris, Scenedesmus basiliensis, Scenedesmus quadricauda, Tetradesmus obliquus
Biochem. J.
300
183-189
1994
-
-
-
-
-
-
1
7
-
7
2
-
-
9
-
-
1
-
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
7
2
-
-
-
-
1
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
643509
Avila
Immunoaffinity chromatography ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CGY 339
Biotechnol. Appl. Biochem.
12
276-283
1990
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
643513
Guranowski
Specific phosphorylase from Eu ...
Acanthamoeba castellanii, Euglena gracilis
Int. J. Biochem.
20
449-455
1988
-
-
-
-
-
-
-
3
-
2
1
-
-
2
-
-
1
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Saccharomyces cerevisiae
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643508
Brevet
Yeast diadenosine 5,5-P1,P4-te ...
Saccharomyces cerevisiae
Biochemistry
26
4763-4768
1987
-
-
-
-
-
-
1
2
-
6
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
6
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643510
Guranowski
Diadenosine 5,5-P1, P4-tetraph ...
Saccharomyces cerevisiae
J. Biol. Chem.
261
5943-5946
1986
-
-
-
-
-
2
8
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
4
-
2
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
643507
Guranowski
Phosphorolytic cleavage of dia ...
Saccharomyces cerevisiae
J. Biol. Chem.
260
3542-3547
1985
-
-
-
-
-
1
4
3
-
8
1
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
8
1
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-