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Literature summary for 2.7.7.49 extracted from

  • Quinones-Mateu, M.E.; Soriano, V.; Domingo, E.; Menendez-Arias, L.
    Characterization of the reverse transcriptase of a human immunodeficiency virus type 1 group O isolate (1997), Virology, 236, 364-373.
    View publication on PubMed
Search for more literature for 2.7.7.49

Application

Application Comment Organism
medicine the recombinant group O enzyme should be useful for studies aimed at discovering and designing drugs directed towards group O isolates of HIV-1 Human immunodeficiency virus 1

Cloned(Commentary)

Cloned (Comment) Organism
cloned from a new HIV-1 group O isolate from Spain and expressed in Escherichia coli Human immunodeficiency virus 1

Inhibitors

Inhibitors Comment Organism Structure
3'-Azido-2',3'-dideoxythymidine 5'-triphosphate inhibits the enzyme from the group M strain BH10 isolate and the enzyme from the Spanish HIV-1 group O isolate Human immunodeficiency virus 1
ddTTP inhibits the enzyme from the group M strain BH10 isolate and the enzyme from the Spanish HIV-1 group O isolate Human immunodeficiency virus 1
loviride IC50: 0.0082-0.16 mM, depending on the substrate used.The enzyme from the group M strain BH10 isolate is sensitive. The enzyme from the Spanish HIV-1 group O isolate shows high-level resistance with IC50 above 0.2 mM Human immunodeficiency virus 1
nevirapine the enzyme from the group M strain BH10 isolate is sensitive, the enzyme from the Spanish HIV-1 group O isolate shows high-level resistance with IC50 above 0.2 mM Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 dTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, enzyme from group O Human immunodeficiency virus 1
0.0067
-
 dTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, enzyme from group M Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cloned from a new HIV-1 group O isolate from Spain and expressed in Escherichia coli Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
dTTP + poly(rA)/(dT)18
-
 Human immunodeficiency virus 1 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 dTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, enzyme from group O Human immunodeficiency virus 1
0.47
-
 dTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, enzyme from group M Human immunodeficiency virus 1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000029
-
 3'-Azido-2',3'-dideoxythymidine 5'-triphosphate pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, group O enzyme Human immunodeficiency virus 1
0.0000059
-
 ddTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, group O enzyme Human immunodeficiency virus 1
0.0000063
-
 3'-Azido-2',3'-dideoxythymidine 5'-triphosphate pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, group M enzyme Human immunodeficiency virus 1
0.000014
-
 ddTTP pH 8.0, 37°C, reaction with poly(rA)*oligo(dT)20, group M enzyme Human immunodeficiency virus 1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
 IC50: 0.0082-0.16 mM, depending on the substrate used.The enzyme from the group M strain BH10 isolate is sensitive. The enzyme from the Spanish HIV-1 group O isolate shows high-level resistance with IC50 above 0.2 mM Human immunodeficiency virus 1 loviride
0.2
-
 the enzyme from the group M strain BH10 isolate is sensitive, the enzyme from the Spanish HIV-1 group O isolate shows high-level resistance with IC50 above 0.2 mM Human immunodeficiency virus 1 nevirapine