Literature summary for 2.7.7.43 extracted from

Matthews, M.M.; McArthur, J.B.; Li, Y.; Yu, H.; Chen, X.; Fisher, A.J.
Catalytic cycle of Neisseria meningitidis CMP-sialic acid synthetase illustrated by high-resolution protein crystallography (2020), Biochemistry, 59, 3157-3168 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
ligand-free and sialic acid-bound enzyme, sitting drop vapor diffusion method, using 0.16 M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 14.4% (w/v) PEG 8000, and 20% (v/v) glycerol. Enzyme in complex with Ca2+ and CTP, sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 6% (w/v) PEG 8000, and 0.2 M calcium acetate	Neisseria meningitidis

Protein Variants

Protein Variants	Comment	Organism
D209A	the mutation is detrimental to enzyme function	Neisseria meningitidis
D211A	the mutation is detrimental to enzyme function	Neisseria meningitidis
E162A	the mutant enzyme shows 14.5% activity compared to the wild type enzyme	Neisseria meningitidis
E162Q	the mutant enzyme shows 15.3% activity compared to the wild type enzyme	Neisseria meningitidis
R165A	the mutant enzyme shows 0.163% activity compared to the wild type enzyme	Neisseria meningitidis

Inhibitors

Inhibitors	Comment	Organism	Structure
2-deoxy-2,3-dehydro-N-acetylneuraminic acid	-	Neisseria meningitidis
Ca2+	-	Neisseria meningitidis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	preferred cofactor, 10 mM used in assay conditions	Neisseria meningitidis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CTP + N-acylneuraminate	Neisseria meningitidis	-	diphosphate + CMP-N-acylneuraminate	-	?

Organism

Organism	UniProt	Comment	Textmining
Neisseria meningitidis	P0A0Z8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Neisseria meningitidis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + N-acylneuraminate	-	Neisseria meningitidis	diphosphate + CMP-N-acylneuraminate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Neisseria meningitidis

Synonyms

Synonyms	Comment	Organism
CMP-sialic acid synthetase	-	Neisseria meningitidis
CSS	-	Neisseria meningitidis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.13	-	at pH 8.5 and 37°C	Neisseria meningitidis	2-deoxy-2,3-dehydro-N-acetylneuraminic acid
8.8	-	at pH 8.5 and 37°C	Neisseria meningitidis	Ca2+

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Release 2023.1 (January 2023)