Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | maximal activation, 210%, at 1 mM | Pelophylax lessonae |
General Stability | Organism |
---|---|
enzyme is stable in lyophilized form | Pelophylax lessonae |
stability can be improved by 1 mM 2-mercaptoethanol | Pelophylax lessonae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2,3-dehydro-N-acetylneuraminate | 4.6 mM, 33% inhibition | Pelophylax lessonae | |
CDP | 1 mM, 49% inhibition | Pelophylax lessonae | |
CMP | 1 mM, 43% inhibition | Pelophylax lessonae | |
diphosphate | 1 mM, 46% inhibition | Pelophylax lessonae | |
NaN3 | 5 mM, 70% inhibition | Pelophylax lessonae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
N-acetylneuraminate | pH 9.0, 37°C | Pelophylax lessonae | |
0.00046 | - |
CTP | pH 9.0, 37°C | Pelophylax lessonae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 40 mM, 30% stimulation | Pelophylax lessonae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax lessonae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Pelophylax lessonae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Pelophylax lessonae | diphosphate + CMP-N-acylneuraminate | - |
? |