General Stability | Organism |
---|---|
stable in the frozen state, each cycle of freezing and thawing leads to a 15% decrease in activity | Pelophylax lessonae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | noncompetitive | Pelophylax lessonae | |
Cu2+ | - |
Pelophylax lessonae | |
EDTA | - |
Pelophylax lessonae | |
Hg2+ | - |
Pelophylax lessonae | |
iodoacetate | - |
Pelophylax lessonae | |
PCMB | - |
Pelophylax lessonae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
CTP | pH 9.0, 37°C | Pelophylax lessonae | |
1.6 | - |
N-acetylneuraminate | pH 9.0, 37°C | Pelophylax lessonae | |
2.3 | - |
N-glycolylneuraminate | pH 9.0, 37°C | Pelophylax lessonae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pelophylax lessonae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal activity in presence of 50 mM Mg2+ or Ca2+ | Pelophylax lessonae | |
Mg2+ | maximal activity in presence of 50 mM Mg2+ or Ca2+ | Pelophylax lessonae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
gel filtration | Pelophylax lessonae |
164000 | - |
1 * 164000, SDS-PAGE | Pelophylax lessonae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax lessonae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pelophylax lessonae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.542 | - |
- |
Pelophylax lessonae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Pelophylax lessonae | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-glycolylneuraminate | - |
Pelophylax lessonae | diphosphate + CMP-N-glycolylneuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 164000, SDS-PAGE | Pelophylax lessonae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pelophylax lessonae |
38 | - |
- |
Pelophylax lessonae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | 42 | 50% of maximal activity at 33°C and at 42°C | Pelophylax lessonae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pelophylax lessonae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
CMP | pH 9.0, 37°C | Pelophylax lessonae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pelophylax lessonae | isoelectric focusing | - |
5.9 |