BRENDA - Enzyme Database show
show all sequences of 2.7.7.41

Distinct properties of the two isoforms of CDP-diacylglycerol synthase

DSouza, K.; Kim, Y.J.; Balla, T.; Epand, R.M.; Biochemistry 53, 7358-7367 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
GTP
small stimulation; small stimulation
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expression in COS-7 cell; expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-alpha-phosphatidylinositol 4,5-bisphosphate
1 mol%, 80% inhibition; strongest inhibitor among the phosphatidylinositols tested
Homo sapiens
L-alpha-phosphatidylinositol 4-phosphate
1 mol%, 50% inhibition
Homo sapiens
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
Homo sapiens
phosphatidylinositol
15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; CDS1 shows no acyl chain-dependent inhibition; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity
Homo sapiens
phosphatidylinositol 4-phosphate
; 55% inhibition at 1 mol%
Homo sapiens
phosphatidylinositol-(4,5)-bisphosphate
80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition; CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C; Km value 1.4 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C; KM value 0.9 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview; 1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
ER tubular localization; ER tubular localization; GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells; GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required; required
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
CTP + phosphatidate
Homo sapiens
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
diphosphate + CDP-diacylglycerol
-
-
?
CTP + phosphatidate
Homo sapiens
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-diacylglycerol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
O95674
-
-
Homo sapiens
Q92903
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
4.5
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
-
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
25% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + phosphatidate
-
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
CTP + phosphatidate
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
CTP + phosphatidate
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
additional information
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
?
-
-
-
-
additional information
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
737701
Homo sapiens
?
-
-
-
-
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
737701
Homo sapiens
?
-
-
-
-
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
737701
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature; assay at room temperature
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at; assay at
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
GTP
small stimulation
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells
Homo sapiens
expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-alpha-phosphatidylinositol 4,5-bisphosphate
strongest inhibitor among the phosphatidylinositols tested
Homo sapiens
L-alpha-phosphatidylinositol 4,5-bisphosphate
1 mol%, 80% inhibition
Homo sapiens
L-alpha-phosphatidylinositol 4-phosphate
1 mol%, 50% inhibition
Homo sapiens
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
Homo sapiens
phosphatidylinositol
CDS1 shows no acyl chain-dependent inhibition
Homo sapiens
phosphatidylinositol
15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity
Homo sapiens
phosphatidylinositol 4-phosphate
-
Homo sapiens
phosphatidylinositol 4-phosphate
55% inhibition at 1 mol%
Homo sapiens
phosphatidylinositol-(4,5)-bisphosphate
CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
phosphatidylinositol-(4,5)-bisphosphate
80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 1.4 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.9 mol%, pH 8.0, 22°C
Homo sapiens
additional information
-
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
additional information
-
additional information
1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
ER tubular localization; GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
endoplasmic reticulum
ER tubular localization; GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
CTP + phosphatidate
Homo sapiens
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
diphosphate + CDP-diacylglycerol
-
-
?
CTP + phosphatidate
Homo sapiens
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-diacylglycerol
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
4.5
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
-
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
25% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + phosphatidate
-
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
CTP + phosphatidate
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
CTP + phosphatidate
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
additional information
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
?
-
-
-
-
additional information
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
737701
Homo sapiens
?
-
-
-
-
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
737701
Homo sapiens
?
-
-
-
-
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
737701
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains; the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
Homo sapiens
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
Homo sapiens
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens
Other publictions for EC 2.7.7.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738826
Qi
CDP-diacylglycerol synthases r ...
Homo sapiens, Mus musculus
J. Lipid Res.
57
767-780
2016
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737701
DSouza
Distinct properties of the two ...
Homo sapiens
Biochemistry
53
7358-7367
2014
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739033
He
Increase in cellular triacylgl ...
Schizosaccharomyces japonicus, Schizosaccharomyces japonicus yFS275, Schizosaccharomyces pombe, Schizosaccharomyces pombe 972
Mol. Biol. Cell
25
4083-4095
2014
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6
7
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739396
Liu
CDP-diacylglycerol synthetase ...
Drosophila melanogaster
PLoS Genet.
10
e1004172
2014
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2
3
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741020
Lilley
The essential roles of cytidin ...
Trypanosoma brucei 927, Trypanosoma brucei brucei
Mol. Microbiol.
92
453-470
2014
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1
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1
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7
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4
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723221
Serricchio
Phosphatidylglycerophosphate s ...
Trypanosoma brucei brucei, Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
Mol. Microbiol.
87
569-579
2013
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1
1
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738000
Tamura
Tam41 is a CDP-diacylglycerol ...
Saccharomyces cerevisiae
Cell Metab.
17
709-718
2013
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2
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1
4
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1
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4
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739305
Zhou
Extraplastidial cytidinediphos ...
Arabidopsis thaliana
Plant J.
75
867-879
2013
1
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1
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1
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4
1
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6
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14
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14
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3
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8
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722903
Waugh
CDP-diacylglycerol phospholipi ...
Homo sapiens
J. Lipid Res.
52
2148-2158
2011
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722419
Shastri
Plasmodium CDP-DAG synthase: a ...
Plasmodium falciparum
Int. J. Parasitol.
40
1257-1268
2010
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691056
Laczko-Dobos
Role of phosphatidylglycerol i ...
Synechocystis sp.
Biochim. Biophys. Acta
1777
1184-1194
2008
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1
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690426
Mercade
Characterization and physical ...
Sus scrofa
Anim. Biotechnol.
18
23-35
2007
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6
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659064
Nishibori
Phosphatidylethanolamine domai ...
Bacillus subtilis, Bacillus subtilis Marburg / DSM 10 / ATCC 6051
J. Bacteriol.
187
2163-2174
2005
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1
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7
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661820
Inglis-Broadgate
Isolation and characterization ...
Mus musculus
Gene
356
19-31
2005
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1
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1
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2
2
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5
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9
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1
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10
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2
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2
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662589
Jiang
Stimulation of cardiac cardiol ...
Mus musculus, Rattus norvegicus
J. Lipid Res.
45
244-252
2004
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2
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643320
Martin
Characterization of Plasmodium ...
Plasmodium falciparum
Mol. Biochem. Parasitol.
110
93-105
2000
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1
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-
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1
-
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-
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1
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643317
Weber
A prototypical cytidylyltransf ...
Bacillus subtilis
Structure Fold. Des.
7
1113-1124
1999
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1
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643318
Weeks
Isolation and expression of an ...
Homo sapiens
DNA Cell Biol.
16
281-289
1997
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1
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1
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5
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1
1
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1
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1
1
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1
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-
643319
Heacock
CDP-diacylglycerol synthase fr ...
Cavia porcellus, Drosophila sp. (in: Insecta), Escherichia coli, Homo sapiens, Rattus norvegicus, Sus scrofa
Biochim. Biophys. Acta
1348
166-172
1997
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1
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2
2
3
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12
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6
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6
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23
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1
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1
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2
2
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12
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6
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23
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1
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643321
Dowhan
CDP-diacylglycerol synthase of ...
Escherichia coli, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1348
157-165
1997
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2
4
1
2
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6
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643322
Monaco
Extraction and stabilization o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
239
166-170
1997
2
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1
1
1
1
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3
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1
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1
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1
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1
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2
1
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1
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643323
Lykidis
The role of CDP-diacylglycerol ...
Homo sapiens
J. Biol. Chem.
272
33402-33409
1997
-
-
1
-
-
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1
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2
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-
-
643324
Kopka
Complementary DNAs encoding eu ...
Arabidopsis thaliana, Solanum tuberosum
Plant Physiol.
113
997-1002
1997
-
-
2
-
-
-
-
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2
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2
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6
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2
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2
2
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2
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2
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2
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2
2
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643325
Saito
Gene cloning and characterizat ...
Rattus norvegicus
J. Biol. Chem.
272
9503-9509
1997
-
-
1
-
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1
3
1
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5
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7
-
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2
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1
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1
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7
-
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2
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-
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-
643328
Shen
Regulation of phospholipid bio ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
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The CDS1 gene encoding CDP-dia ...
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643316
Wu
Regulation of PLC-mediated sig ...
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Characterization of cytidine t ...
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CDPdiacylglycerol synthase fro ...
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643302
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CDP-diacylglycerol synthesis i ...
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Bovine brain microsomal CDP-di ...
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Specific inhibition of phospha ...
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CDP-diacylglycerol synthase ac ...
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Biosynthesis of CDP-diacylglyc ...
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Liteplo
The stimulation of rat liver m ...
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Belendiuk
CTP-phosphatidic acid cytidylt ...
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Partial purification and prope ...
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The influence of exogenous and ...
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Sribney
Studies on the synthesis of CD ...
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Sturton
Factors controlling the activi ...
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Bishop
Studies on the formation by ra ...
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Biosynthesis of cytidine dipho ...
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Petzold
The biosynthesis of cytidine d ...
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