Any feedback?
Literature summary for 2.7.7.4 extracted from
- Rat liver ATP-sulfurylase: purification, kinetic characterization, and interaction with arsenate, selenate, phosphate, and other inorganic oxyanions (1989), Arch. Biochem. Biophys., 269, 156-174.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ClO3- | competitive with SO42- and apparently uncompetitive with respect to MgATP2- | Rattus norvegicus |  |
| ClO4- | competitive with SO42- and apparently uncompetitive with respect to MgATP2- | Rattus norvegicus | |
| diphosphate | mixed-type inhibitor with respect to both MgATP2- and MoO42- | Rattus norvegicus | |
| FSO3- | competitive with SO42- and apparently uncompetitive with respect to MgATP2- | Rattus norvegicus | |
| NO3- | competitive with SO42- and apparently uncompetitive with respect to MgATP2- | Rattus norvegicus | |
| S2O32- | noncompetitive mixed-type inhibition with respect to MgATP2- | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
ATP | 30°C, pH 8.0, reaction with CrO42- | Rattus norvegicus | |
| 0.012 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.019 | - |
ATP | 30°C, pH 8.0, reaction with SeO42- | Rattus norvegicus | |
| 0.019 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.1 | - |
SeO42- | 30°C, pH 8.0 | Rattus norvegicus | |
| 0.12 | - |
CrO42- | 30°C, pH 8.0 | Rattus norvegicus | |
| 0.13 | - |
ATP | 30°C, pH 8.0, reaction with MoO42- | Rattus norvegicus | |
| 0.13 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.18 | - |
SO42- | 30°C, pH 8.0 | Rattus norvegicus | |
| 0.21 | - |
ATP | 30°C, pH 8.0, reaction with SO42- | Rattus norvegicus | |
| 0.21 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.24 | - |
MoO42- | 30°C, pH 8.0 | Rattus norvegicus | |
| 0.27 | - |
ATP | 30°C, pH 8.0, reaction with WO42- | Rattus norvegicus | |
| 0.27 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.47 | - |
WO42- | 30°C, pH 8.0 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
2 * 62000, SDS-PAGE | Rattus norvegicus |
| 122000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | Rattus norvegicus | first enzyme of the two-step sulfate activation sequence | diphosphate + adenylylsulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.2 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + chromate | - |
Rattus norvegicus | diphosphate + adenylyl chromate | - |
r | |
| ATP + CrO42- | - |
Rattus norvegicus | AMP + adenylyl-chromate | followed by nonenzymatic reaction of adenylylmolybdate with H2O to AMP and molybdate | ? | |
| ATP + molybdate | - |
Rattus norvegicus | diphosphate + adenylyl molybdate | - |
r | |
| ATP + MoO42- | - |
Rattus norvegicus | AMP + adenylylmolybdate | - |
? | |
| ATP + selenate | - |
Rattus norvegicus | diphosphate + adenylyl selenate | - |
r | |
| ATP + SeO42- | 20% of the activity with SO42- | Rattus norvegicus | AMP + adenylylselenate | reaction is followed by nonenzymatic reaction of adenylylselenate with H2O to AMP and SeO42- | ? | |
| ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | first enzyme of the two-step sulfate activation sequence | Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylyl sulfate | - |
r | |
| ATP + tungstate | - |
Rattus norvegicus | diphosphate + adenylyl tungstate | - |
r | |
| ATP + WO42- | - |
Rattus norvegicus | AMP + adenylyl-wolframate | followed by nonenzymatic reaction of adenylyl-WO42- with H2O to AMP and WO42- | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | Tris-MES buffer | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
diphosphate | pH 8.0, 30°C, at 20 mM SO42- and 5 mM excess Mg2+ | Rattus norvegicus | |
| 0.033 | - |
ClO3- | pH 8.0, 30°C | Rattus norvegicus | |
| 0.28 | - |
ClO4- | pH 8.0, 30°C | Rattus norvegicus | |
| 0.5 | - |
FSO3- | pH 8.0, 30°C | Rattus norvegicus | |
| 1.13 | - |
S2O32- | pH 8.0, 30°C | Rattus norvegicus | |
| 1.2 | - |
NO3- | pH 8.0, 30°C | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
