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Literature summary for 2.7.7.33 extracted from
- Cloning, sequencing, and overexpression in Escherichia coli of the alpha-D-glucose-1-phosphate cytidylyltransferase gene isolated from Yersinia pseudotuberculosis (1994), J. Bacteriol., 176, 1840-1849.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Yersinia pseudotuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C | Yersinia pseudotuberculosis |  |
| 0.082 | - |
CTP | pH 7.5, 37°C | Yersinia pseudotuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ | Yersinia pseudotuberculosis | |
| Mg2+ | with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ | Yersinia pseudotuberculosis | |
| Mn2+ | with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ | Yersinia pseudotuberculosis | |
| additional information | no activation by Cu2+ | Yersinia pseudotuberculosis | |
| additional information | no activation by Ni2+ | Yersinia pseudotuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
4 * 29000, SDS-PAGE | Yersinia pseudotuberculosis |
| 116000 | - |
gel filtration | Yersinia pseudotuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + D-glucose 1-phosphate | Yersinia pseudotuberculosis | first step in biosynthesis of CDP-ascarylose | ? | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yersinia pseudotuberculosis | Q57430 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.51 | - |
- |
Yersinia pseudotuberculosis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C stable for months with no loss of activity, freezing and thawing does not affect the enzyme activity | Yersinia pseudotuberculosis |
| 4°C stable for days | Yersinia pseudotuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + alpha-D-glucose 1-phosphate | - |
Yersinia pseudotuberculosis | diphosphate + CDPglucose | - |
r | |
| CTP + D-glucose 1-phosphate | first step in biosynthesis of CDP-ascarylose | Yersinia pseudotuberculosis | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 29000, SDS-PAGE | Yersinia pseudotuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Yersinia pseudotuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Yersinia pseudotuberculosis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
irreversible inactivation below pH 6 | Yersinia pseudotuberculosis |
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