Application | Comment | Organism |
---|---|---|
analysis | DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates. Amino- and nitrophenyl-modified dNTPs are good substrates giving 3'-end stretches of different lengths depending on the nucleotide and concentration. d[7-Deaza-7-(3-nitrophenyl)]GTP is efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide, resulting in a considerable enhancement of voltammetric signals due to the nitro group reduction. Discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes is easily accomplished, and tumour suppressor p53 protein is able to recognize a specific binding site within tail-labelled DNA substrates | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P06526 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
d[5-(3-aminophenyl)]CTP + DNA | 5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[5-(3-aminophenyl)]UTP + DNA | 5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[5-(3-nitrophenyl)]CTP + DNA | 5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[5-(3-nitrophenyl)]UTP + DNA | 5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[7-deaza-7-(3-aminophenyl)]ATP + DNA | the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[7-deaza-7-(3-aminophenyl)]GTP + DNA | - |
Bos taurus | diphosphate + ? | - |
? | |
d[7-deaza-7-(3-nitrophenyl)]ATP + DNA | the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides | Bos taurus | diphosphate + ? | - |
? | |
d[7-deaza-7-(3-nitrophenyl)]GTP + DNA | most useful substrate, efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide | Bos taurus | diphosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TdT | - |
Bos taurus |