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Literature summary for 2.7.7.30 extracted from

  • Wang, H.; Zhang, C.; Yang, Q.; Feng, J.; Chen, H.; Gu, Z.; Zhang, H.; Chen, W.; Chen, Y.
    Production of GDP-L-fucose from exogenous fucose through the salvage pathway in Mortierella alpina (2016), RSC Adv., 6, 46308-46316 .
No PubMed abstract available
Search for more literature for 2.7.7.30

Activating Compound

Activating Compound Comment Organism Structure
L-fucose
-
 Mortierella alpina

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Gold cells Mortierella alpina

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Mortierella alpina
Mg2+ Mg2+ is inhibitory at concentrations higher than 4 mM Mortierella alpina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 beta-L-fucose 1-phosphate at pH 7.0 and 30°C Mortierella alpina

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity at 4 mM. No activity can be detected when the Mg2+ concentration is below 1 nM Mortierella alpina
additional information K+, Mn2+, Co2+, Ca2+, Cu2+, Zn2+, Ni2+, Hg2+, and Fe2+ have little effect on the enzyme activity Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + beta-L-fucose 1-phosphate Mortierella alpina
-
 diphosphate + GDP-L-fucose
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alpina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel ion affinity column chromatography Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + beta-L-fucose 1-phosphate
-
 Mortierella alpina diphosphate + GDP-L-fucose
-
 ?

Subunits

Subunits Comment Organism
? x * 69000, SDS-PAGE Mortierella alpina

Synonyms

Synonyms Comment Organism
GDP-L-fucose pyrophosphorylase
-
 Mortierella alpina
GFPP
-
 Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Mortierella alpina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
12 50 the enzyme retains 40% of its initial activity after 1.5 h of exposure at 12°C, and loses more than 50% of its initial activity after 1 h of exposure at 20°C, 28°C and 37°C. The enzyme loses nearly 100% of its activity after 30 min of exposure above 50°C Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mortierella alpina

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12 less than 30% enzyme activity is retained after incubation at pH 3.0 and pH 12.0 at 4°C for 2 h Mortierella alpina

pI Value

Organism Comment pI Value Maximum pI Value
Mortierella alpina calculated from amino acid sequence
-
 5.9