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Literature summary for 2.7.7.30 extracted from

  • Liu, Y.; Hu, H.; Wang, J.; Zhou, Q.; Wu, P.; Yan, N.; Wang, H.; Wu, J.; Sun, L.
    Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis (2019), Protein Cell, 10, 365-369 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.30

Protein Variants

Protein Variants Comment Organism
A723R the mutant shows about 75% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
D594A the mutant shows about 55% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
D601A the mutant shows about 40% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
D762A the mutant shows about 85% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G597A the mutant shows about 20% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G598A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G713A the mutant shows about 125% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G715A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G717A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G757A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G758A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G759A the mutant shows about 85% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G75A the mutant shows about 60% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G76A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G88A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
G899A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G901A the mutant shows about 87% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
G902A the mutant shows about 80% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
H73A the mutant shows about 40% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
I721A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
K89A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
L722A the mutant shows about 120% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
P711A the mutant shows about 120% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
R592A the mutant shows about 110% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
R80A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
R82A the mutant shows about 50% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
S714A the mutant shows about 130% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
S719A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
S720A the mutant shows about 75% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
S78A the mutant shows about 80% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
T602A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
T718A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
338000
-
 ultracentrifugation sedimentation velocity analysis Bacteroides fragilis
440159
-
 gel filtration Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + beta-L-fucose 1-phosphate Bacteroides fragilis
-
 diphosphate + GDP-L-fucose
-
 r
GTP + beta-L-fucose 1-phosphate Bacteroides fragilis 9343
-
 diphosphate + GDP-L-fucose
-
 r

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides fragilis 9343
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis diphosphate + GDP-L-fucose
-
 r
GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis 9343 diphosphate + GDP-L-fucose
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 105000, calculated from amino acid sequence Bacteroides fragilis

Synonyms

Synonyms Comment Organism
FKP bifunctional enzyme with L-fucokinase and GDP-fucose pyrophosphorylase activities Bacteroides fragilis
GDP-fucose pyrophosphorylase
-
 Bacteroides fragilis
GFPP
-
 Bacteroides fragilis