Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | - |
Ostreococcus tauri |
Cloned (Comment) | Organism |
---|---|
the S and L subunit genes are inserted into the pSC-B vector using the StrataClone Blunt PCR cloning kit, S ann L subunits are subcloned into pMAB5 and pMAB6 expression vectors, respectively, for expression in Escherichia coli AC7OR1-504 cells | Ostreococcus tauri |
Protein Variants | Comment | Organism |
---|---|---|
OtaL_D171A | mutant subunit L | Ostreococcus tauri |
OtaL_K224R | mutant subunit L | Ostreococcus tauri |
OtaS_D148A | mutant subunit S | Ostreococcus tauri |
OtaS_D148A/K201R | double mutant subunit S | Ostreococcus tauri |
OtaS_K201R | mutant subunit S | Ostreococcus tauri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Ostreococcus tauri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ostreococcus tauri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
S subunit, determined by SDS-PAGE | Ostreococcus tauri |
52000 | - |
L subunit, determined by SDS-PAGE | Ostreococcus tauri |
204000 | - |
heterotetramer, S2L2, calculated | Ostreococcus tauri |
222000 | - |
heterotetramer, S2L2, determined by gel filtration | Ostreococcus tauri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Ostreococcus tauri | - |
diphosphate + ADP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ostreococcus tauri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a DEAE-Sepharose Fast Flow weak anion exchange and a Source 15Q 4.6/100 PE strong anion exchange column, and a Resource PHE 1-ml HIC hydrophobic column | Ostreococcus tauri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Ostreococcus tauri | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | the enzyme includes two distinct S and L homologous subunits, S2L2 or alpha2beta2 | Ostreococcus tauri |
Synonyms | Comment | Organism |
---|---|---|
ADP-Glc PPase | - |
Ostreococcus tauri |
ADP-glucose pyrophosphorylase | - |
Ostreococcus tauri |
OtaL | Ostreococcus tauri ADP-glucose pyrophosphorylase subunit L | Ostreococcus tauri |
OtaS | Ostreococcus tauri ADP-glucose pyrophosphorylase subunit S | Ostreococcus tauri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Ostreococcus tauri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Ostreococcus tauri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ostreococcus tauri |
General Information | Comment | Organism |
---|---|---|
physiological function | ADP-glucose pyrophosphorylase controls starch synthesis in plants | Ostreococcus tauri |