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Literature summary for 2.7.7.19 extracted from

  • Hu, J.L.; Liang, H.; Zhang, H.; Yang, M.Z.; Sun, W.; Zhang, P.; Luo, L.; Feng, J.X.; Bai, H.; Liu, F.; Zhang, T.; Yang, J.Y.; Gao, Q.; Long, Y.; Ma, X.Y.; Chen, Y.; Zhong, Q.; Yu, B.; Liao, S.; Wang, Y.; Zhao, Y.; Zeng, M.S.; Cao, N.; Wang, J.; Chen, W.; Yang, H.T.; Gao, S.
    FAM46B is a prokaryotic-like cytoplasmic poly(A) polymerase essential in human embryonic stem cells (2020), Nucleic Acids Res., 48, 2733-2748 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens
expressed in Escherichia coli Rosetta (DE3) cells Xenopus tropicalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2% (w/v) tacsimate pH 5.0, 0.15 M sodium citrate tribasic dehydrate pH 5.6 and 10% (w/v) PEG 3350 at 10°C Xenopus tropicalis

Protein Variants

Protein Variants Comment Organism
D124A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
D126A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
E200A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
G107A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
S108A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Xenopus tropicalis 5737
-
nucleus
-
 Homo sapiens 5634
-
nucleus
-
 Xenopus tropicalis 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 4 mM used in assay conditions. When Mg2+ is replaced by Mn2+, the protein becomes more potent in catalyzing polyadenylation Homo sapiens
Mg2+ 4 mM used in assay conditions. When Mg2+ is replaced by Mn2+, the protein becomes more potent in catalyzing polyadenylation Xenopus tropicalis
Mn2+ 4 mM used in assay conditions Homo sapiens
Mn2+ 4 mM used in assay conditions Xenopus tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNAn Homo sapiens
-
 diphosphate + RNAn+1
-
 ?
ATP + RNAn Xenopus tropicalis
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96A09
-
-
Xenopus tropicalis F7E7M3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens
Ni-NTA column chromatography and Superdex 200 gel filtration Xenopus tropicalis

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Homo sapiens
-
embryonic stem cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA (A)15
-
 Homo sapiens diphosphate + RNA(A)16
-
 ?
ATP + RNA (A)15
-
 Xenopus tropicalis diphosphate + RNA(A)16
-
 ?
ATP + RNAn
-
 Homo sapiens diphosphate + RNAn+1
-
 ?
ATP + RNAn
-
 Xenopus tropicalis diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
FAM46B
-
 Homo sapiens
FAM46B
-
 Xenopus tropicalis
PAP
-
 Homo sapiens
PAP
-
 Xenopus tropicalis
poly(A) polymerase
-
 Homo sapiens
poly(A) polymerase
-
 Xenopus tropicalis

Expression

Organism Comment Expression
Homo sapiens the enzyme is highly expressed in human pre-implantation embryos and pluripotent stem cells up

General Information

General Information Comment Organism
malfunction enzyme knock-out is lethal. Knock-down of enzyme induces apoptosis and restricts protein synthesis Homo sapiens
physiological function the enzyme is indispensable for the viability of human embryonic stem cells Homo sapiens