Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | above 0.5 mM | Artemia sp. | |
K+ | 80 mM: 50% inhibition; KCl | Artemia sp. | |
NH4+ | 50 mM: 50% inhibition; ammonium sulfate | Artemia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
ATP | 30°C, pH 8.5 | Artemia sp. | |
0.06 | - |
dATP | 30°C, pH 8.5 | Artemia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Artemia sp. | |
Mn2+ | optimal concentration: 2-4 mM | Artemia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Artemia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | Artemia sp. | processing and activation of stored mRNAs after resumption of development | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Artemia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | encysted, dormant | Artemia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Artemia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | primer required | Artemia sp. | diphosphate + RNA(A)n | length of the poly(A) tail is dependent on incubation time and RNA primer concentration | ? | |
ATP + RNA | no specificity for the 3'-terminal nucleotides | Artemia sp. | diphosphate + RNA(A)n | length of the poly(A) tail is dependent on incubation time and RNA primer concentration | ? | |
ATP + RNA | processing and activation of stored mRNAs after resumption of development | Artemia sp. | ? | - |
? | |
dATP + RNA | 15% of the activity with ATP | Artemia sp. | diphosphate + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, stable | Artemia sp. |
45 | - |
5 min, 50% loss of activity | Artemia sp. |
50 | - |
5 min, complete inactivation | Artemia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Artemia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7: about 40% of maximum activity, pH 8-8.5: maximum activity | Artemia sp. |