Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F171L | site-directed mutagenesis, 10fold decreased activity | Homo sapiens |
F171S | site-directed mutagenesis, no activity | Homo sapiens |
F171W | site-directed mutagenesis, severely reduced abundance | Homo sapiens |
F171Y | site-directed mutagenesis, 4% activity compared to wild-type, no inhibition by excess UDP-glucose | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP-glucose | at high concentrations, wild-type and mutants F171L and F171S, not F171Y | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
native mutant F171S | - |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant wild-type and mutants F171Y and F171L to near homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | modeling of the human enzyme structure onto the crystal structure of the Escherichia coli enzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GALT | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, pH 8.7 | Homo sapiens | |
9.3 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, pH 8.7 | Homo sapiens | |
98 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, pH 8.7 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
assay at | Homo sapiens |