Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
dissociation of the native dimeric species of the enzyme occurs at an urea concentration of around 4 M, leading to the accumulation of partially folded monomers that unfold totally at urea concentrations above 5.5 M | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-azido-3'-deoxythymidine 5'-monophosphate | potent competitive inhibitor | Mycobacterium tuberculosis | |
3'-azido-3'-deoxythymidine monophosphate | - |
Mycobacterium tuberculosis | |
5-methyl iso-dCMP | competitive | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
dTMP | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.1 | - |
ATP | pH 7.4, 30°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22635 | - |
2 * 22635, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
46600 | - |
sedimentation equilibrium ultracentrifugation | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | - |
Mycobacterium tuberculosis | ADP + dTDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22635, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, 50% inactivation, irreversible | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
3'-azido-3'-deoxythymidine monophosphate | pH 7.4, 30°C | Mycobacterium tuberculosis | |
0.13 | - |
5-methyl iso-dCMP | pH 7.4, 30°C | Mycobacterium tuberculosis |