Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-iodo-2'-dUMP | - |
Escherichia coli | |
dCTP | - |
Escherichia coli | |
dTTP | - |
Escherichia coli | |
dUMP | - |
Escherichia coli | |
NaCl | 0.1 M, 25% inhibition | Escherichia coli | |
NH4Cl | 0.4 M, 75% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
dTMP | pH 7.8, 37°C | Escherichia coli | |
1.2 | - |
ATP | pH 7.8, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 18% of maximal activation with Mg2+ | Escherichia coli | |
Mg2+ | absolute requirement for divalent cation. When Mg2+ is equal to ATP, the rate of dTMP kinase reaction is maximal | Escherichia coli | |
Mn2+ | 41% of maximal activation with Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration, sucrose density gradient centrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 1 year, 20-30% loss of activity | Escherichia coli |
2°C, half-life of about 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-iodo-2'-dUMP | 60% of the maximal activity with dTMP | Escherichia coli | ADP + 5-iodo-2'-dUDP | - |
? | |
ATP + dTMP | - |
Escherichia coli | ADP + dTDP | - |
? | |
ATP + dTMP | - |
Escherichia coli B / ATCC 11303 | ADP + dTDP | - |
? | |
ATP + dUMP | at 15% of the activity with dTMP | Escherichia coli | ADP + dUDP | - |
? | |
ATP + dUMP | at 15% of the activity with dTMP | Escherichia coli B / ATCC 11303 | ADP + dUDP | - |
? | |
CTP + dTMP | about 30% of the activity with ATP | Escherichia coli | CDP + dTDP | - |
? | |
CTP + dTMP | about 30% of the activity with ATP | Escherichia coli B / ATCC 11303 | CDP + dTDP | - |
? | |
dATP + dTMP | about 80% of the activity with ATP | Escherichia coli | dADP + dTDP | - |
? | |
dATP + dTMP | about 80% of the activity with ATP | Escherichia coli B / ATCC 11303 | dADP + dTDP | - |
? | |
dCTP + dTMP | about 20% of the activity with ATP | Escherichia coli | dCDP + dTDP | - |
? | |
dGTP + dTMP | about 10% of maximal activity with ATP | Escherichia coli | dGDP + dTDP | - |
? | |
dGTP + dTMP | about 10% of maximal activity with ATP | Escherichia coli B / ATCC 11303 | dGDP + dTDP | - |
? | |
dTTP + dTMP | about 3% of the activity with ATP | Escherichia coli | dTDP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.7 | pH 7.0: about 50% of maximal activity, pH 8.7: about 40% of maximal activity | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
5-iodo-2'-dUMP | pH 7.8, 37°C | Escherichia coli |