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Literature summary for 2.7.4.9 extracted from

  • Chen, M.C.; Walker, J.; Prusoff, W.H.
    Kinetic studies of herpes simplex virus type 1-encoded thymidine and thymidylate kinase, a multifunctional enzyme (1979), J. Biol. Chem., 254, 10747-10753.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-iodo-5'-fluoro-2',5'-dideoxyuridine
-
Human alphaherpesvirus 1
5-iodo-dUMP competitive versus both thymidylate and thymidine Human alphaherpesvirus 1
ADP ADP*Mg2+ product inhibition, competitive versus MgATP2-, noncompetitive versus thymidylate Human alphaherpesvirus 1
dTDP product inhibition, competitive versus MgATP2- Human alphaherpesvirus 1
dTTP competitive versus both thymidine and thymidylate Human alphaherpesvirus 1
MgADP-
-
Human alphaherpesvirus 1

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
thymidine/thymidylate multifunctional kinase
-

Reaction

Reaction Comment Organism Reaction ID
ATP + dTMP = ADP + dTDP addition of substrates to the enzyme is random, while release of the products is ordered Human alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information thymidine/thymidylate multifunctional kinase Human alphaherpesvirus 1 ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
5-iodo-5'-fluoro-2',5'-dideoxyuridine versus thymidylate Human alphaherpesvirus 1
0.0009
-
5-iodo-5'-fluoro-2',5'-dideoxyuridine versus thymidine Human alphaherpesvirus 1
0.05
-
dTTP verus thymidylate Human alphaherpesvirus 1
0.059
-
dTDP versus thymidylate Human alphaherpesvirus 1
0.06
-
MgADP- versus ATP*Mg2+ Human alphaherpesvirus 1
0.062
-
dTTP versus thymidine Human alphaherpesvirus 1
0.1
-
MgADP- versus thymidylate Human alphaherpesvirus 1