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Literature summary for 2.7.4.9 extracted from

  • Jong, A.Y.S.; Campbell, J.L.
    Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. General properties, kinetic analysis, and subcellular localization (1984), J. Biol. Chem., 259, 14394-14398.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 0.35 M, 50% inhibition Saccharomyces cerevisiae
5-iodo-dUMP 2.5 mM, 30% inhibition Saccharomyces cerevisiae
ADP 2.5 mM, 24% inhibition Saccharomyces cerevisiae
dADP 2.5 mM, 23% inhibition Saccharomyces cerevisiae
dTDP 2.5 mM, 75% inhibition Saccharomyces cerevisiae
dTTP 2.5 mM, 17% inhibition Saccharomyces cerevisiae
dUDP 2.5 mM, 15% inhibition Saccharomyces cerevisiae
dUMP 2.5 mM, 19% inhibition Saccharomyces cerevisiae
KCl 0.5 M, 10% inhibition Saccharomyces cerevisiae
thymidine 2.5 mM, 40% inhibition Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Saccharomyces cerevisiae 5737
-
mitochondrion no activity in mitochondria Saccharomyces cerevisiae 5739
-
nucleus
-
Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, Co2 can fully substitute for Mg2+ Saccharomyces cerevisiae
Fe2+ divalent cation required, Fe2+ can fully substitute for Mg2+ Saccharomyces cerevisiae
Mg2+ divalent cation required Saccharomyces cerevisiae
Mn2+ divalent cation required, Mn2+ can fully substitute for Mg2+ Saccharomyces cerevisiae
NaCl from 0-0.5 M does not affect activity Saccharomyces cerevisiae
Ni2+ divalent cation required, Ni2+ can fully substitute for Mg2+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-iodo-dUMP 34% of the activity with dTMP Saccharomyces cerevisiae ADP + 5-iodo-dUDP
-
?
ATP + dTMP
-
Saccharomyces cerevisiae ADP + dTDP
-
?
ATP + dUMP 31% of the activity with dTMP Saccharomyces cerevisiae ADP + dUDP
-
?
CTP + dTMP 26% of the activity with ATP Saccharomyces cerevisiae CDP + dTDP
-
?
dATP + dTMP 92% of the activity with ATP Saccharomyces cerevisiae dADP + dTDP
-
?
dCTP + dTMP 49% of the activity ATP Saccharomyces cerevisiae dCDP + dTDP
-
?
dGTP + dTMP 42% of the activity with ATP Saccharomyces cerevisiae dGDP + dTDP
-
?
GTP + dTMP 35% of the activity with ATP Saccharomyces cerevisiae GDP + dTDP
-
?
UTP + dTMP 36% of the activity with ATP Saccharomyces cerevisiae UDP + dTDP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, in absence of substrate activity decreases to 32% of the initial activity, completely stable in presence of 10 mM dTMP Saccharomyces cerevisiae
60
-
10 min, in absence of substrate activity decreases to 1% of the initial activity, partial protection in presence of 10 mM dTMP Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5: about 45% of maximal activity, pH 6-9: optimum Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.62
-
dTDP pH 8.0, 37°C Saccharomyces cerevisiae