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Literature summary for 2.7.4.9 extracted from

  • Kielley, R.K.
    Purification and properties of thymidine monophosphate kinase from mouse hepatoma (1970), J. Biol. Chem., 245, 4204-4212.
    View publication on PubMed

General Stability

General Stability Organism
dTDP and dTTP are effective as enzyme stabilizer Mus musculus
the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.4 mM Mus musculus
ADP 100 nM, 64% inhibition Mus musculus
Ca2+
-
Mus musculus
CH2ATP 100 nM, 23% inhibition Mus musculus
dADP 100 nM, 57% inhibition Mus musculus
dATP 100 nM, 15% inhibition Mus musculus
dGTP 100 nM, 12% inhibition Mus musculus
dTDP 100 nM, 39% inhibition Mus musculus
GDP 100 nM, 21% inhibition Mus musculus
Mn2+ slight inhibition Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
dTMP pH 7.4, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, concentration range for maximal activity is From Mg:ATP ratios of 1 to 3 with an optimum around 1.3 to 1.5 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma ascites cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.76
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dTMP
-
Mus musculus ADP + dTDP
-
r
ATP + dUMP 23% of the activity with dTMP Mus musculus ADP + dUDP
-
r
dATP + dTMP 70.1% of the activity with ATP Mus musculus dADP + dTDP
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
ADP pH 7.4, 37°C Mus musculus