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Literature summary for 2.7.4.6 extracted from
- Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase (2020), FEBS J., 288, 2398-2417 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion | Aspergillus fumigatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.402 | - |
GTP | pH 8.0, 30°C | Aspergillus fumigatus |  |
| 0.513 | - |
ATP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 0.617 | - |
ITP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 1.32 | - |
CTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 1.35 | - |
UTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 2.23 | - |
TTP | pH 8.0, 30°C | Aspergillus fumigatus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q7Z8P9 | - |
- |
| Aspergillus fumigatus ATCC MYA-4609 | Q7Z8P9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ATP | - |
Aspergillus fumigatus | ATP + ADP | - |
? | |
| ADP + ATP | - |
Aspergillus fumigatus ATCC MYA-4609 | ATP + ADP | - |
? | |
| ADP + CTP | - |
Aspergillus fumigatus | ATP + CDP | - |
? | |
| ADP + CTP | - |
Aspergillus fumigatus ATCC MYA-4609 | ATP + CDP | - |
? | |
| ADP + GTP | - |
Aspergillus fumigatus | ATP + GDP | - |
? | |
| ADP + GTP | - |
Aspergillus fumigatus ATCC MYA-4609 | ATP + GDP | - |
? | |
| ADP + ITP | - |
Aspergillus fumigatus | ATP + IDP | - |
? | |
| ADP + TTP | - |
Aspergillus fumigatus | ATP + TDP | - |
? | |
| ADP + UTP | - |
Aspergillus fumigatus | ATP + UDP | - |
? | |
| ADP + UTP | - |
Aspergillus fumigatus ATCC MYA-4609 | ATP + UDP | - |
? | |
| additional information | adenine nucleosides are used preferentially compared to cytidine nucleosides | Aspergillus fumigatus | ? | - |
- |
|
| additional information | adenine nucleosides are used preferentially compared to cytidine nucleosides | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | based on crystallographic symmetry, the enzyme forms a hexamer which is arranged as a trimer of dimers | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Afu5g03490 | - |
Aspergillus fumigatus |
| NDK | - |
Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53.1 | - |
CTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 83.2 | - |
ITP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 140 | - |
UTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 178 | - |
TTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 188 | - |
GTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 321 | - |
ATP | pH 8.0, 30°C | Aspergillus fumigatus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| drug target | as an essential enzyme in Aspergillus fumigatus, nucleoside-diphosphate kinase is an attractive target for antifungals | Aspergillus fumigatus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40.2 | - |
CTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 79.8 | - |
TTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 104 | - |
UTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 135 | - |
ITP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 468 | - |
GTP | pH 8.0, 30°C | Aspergillus fumigatus | |
| 626 | - |
ATP | pH 8.0, 30°C | Aspergillus fumigatus | |
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