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Literature summary for 2.7.4.6 extracted from

  • Dautant, A.; Henri, J.; Wales, T.E.; Meyer, P.; Engen, J.R.; Georgescauld, F.
    Remodeling of the binding site of nucleoside diphosphate kinase revealed by X-ray structure and H/D exchange (2019), Biochemistry, 58, 1440-1449 .
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D93N less stable than wild-type enzyme. The Tm-value of the mutant enzyme is 48.4°C, compared to 76.1°C for the wild-type enzyme Mycobacterium tuberculosis
R80A less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. Compensatory stabilizing mechanisms appear for R80A mutant. The R80A mutant crystallizes into space group I222 that is unusual for nucleoside diphosphate kinases, and its hexameric structure reveals the occurrence at the trimer interface of a stabilizing hydrophobic patch around the mutation. The Tm-value of the mutant enzyme is 69.0°C, compared to 76.1°C for the wild-type enzyme Mycobacterium tuberculosis
R80N less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. The Tm-value of the mutant enzyme is 69.3 °C, compared to 76.1°C for the wild-type enzyme Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis A5U5E1
-
-
Mycobacterium tuberculosis ATCC 25177 A5U5E1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 8-bromoinosine 5'-diphosphate
-
 Mycobacterium tuberculosis ADP + 8-bromoinosine 5'-triphosphate
-
 ?
ATP + 8-bromoinosine 5'-diphosphate
-
 Mycobacterium tuberculosis ATCC 25177 ADP + 8-bromoinosine 5'-triphosphate
-
 ?