Cloned (Comment) | Organism |
---|---|
expression of GST-tagged NPDK2 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H197C | NDPK2 kinase site mutant | Arabidopsis thaliana |
H197C | no enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 | Arabidopsis thaliana |
L225stop | C-terminal deletion mutant | Arabidopsis thaliana |
L225stop | increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 | Arabidopsis thaliana |
LP175S | no interaction between NDPK2 and small G proteins, EC 3.6.5.2 | Arabidopsis thaliana |
P175S | NDPK2 kinase site mutant | Arabidopsis thaliana |
P175S | stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, with a slight reduction compared to wild-type NDPK2 | Arabidopsis thaliana |
R230stop | C-terminal deletion mutant | Arabidopsis thaliana |
R230stop | increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2 | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | O64903 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins | Arabidopsis thaliana | ? | - |
? | |
additional information | NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
NDPK2 | - |
Arabidopsis thaliana |
nucleoside diphosphate kinase | - |
Arabidopsis thaliana |
nucleoside diphosphate kinase-2 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |