Literature summary for 2.7.3.2 extracted from

Weinsanto, I.; Mouheiche, J.; Laux-Biehlmann, A.; Delalande, F.; Marquette, A.; Chavant, V.; Gabel, F.; Cianferani, S.; Charlet, A.; Parat, M.O.; Goumon, Y.
Morphine binds creatine kinase B and inhibits its activity (2018), Front. Cell. Neurosci., 12, 464 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
morphine	0.00001-0.1 mM morphine significantly reduces recombinant enzymatic activity (27% inhibition at 0.001 mM, 80% inhibition at more than 0.05 mM); 27% inhibition at 0.001 m, 80% inhibition at more than 0.05 mM	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
84000	-	gel filtration	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + phosphocreatine	Mus musculus	-	ATP + creatine	-	r
ATP + creatine	Mus musculus	-	ADP + phosphocreatine	-	r

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q04447	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Hitrap column chromatography	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
astrocyte	-	Mus musculus	-
brain	-	Mus musculus	-
neuron	-	Mus musculus	-
oligodendrocyte	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphocreatine	-	Mus musculus	ATP + creatine	-	r
ATP + creatine	-	Mus musculus	ADP + phosphocreatine	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 42000, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
CK-B	-	Mus musculus
creatine kinase B	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

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Release 2023.1 (January 2023)