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Literature summary for 2.7.3.2 extracted from
- Two cryptosporidia species encode active creatine kinases that are not seen in other apicomplexa species (2020), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 246-247, 110459 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Cryptosporidium andersoni |
| expressed in Escherichia coli BL21(DE3) cells | Cryptosporidium muris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cryptosporidium muris |  |
| Creatine | - |
Cryptosporidium muris | |
| glycocyamine | - |
Cryptosporidium muris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
ATP | in form of Mg-ATP, at pH 8.5 and 25°C | Cryptosporidium muris | |
| 1.7 | - |
ATP | in form of Mg-ATP, at pH 8.5 and 25°C | Cryptosporidium muris | |
| 2 | - |
Creatine | at pH 8.5 and 25°C | Cryptosporidium muris | |
| 6.7 | - |
glycocyamine | at pH 8.5 and 25°C | Cryptosporidium muris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Cryptosporidium muris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Cryptosporidium muris | - |
ADP + phosphocreatine | - |
? | |
| ATP + creatine | Cryptosporidium andersoni | - |
ADP + phosphocreatine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptosporidium andersoni | - |
- |
- |
| Cryptosporidium andersoni | A0A1J4MR22 | - |
- |
| Cryptosporidium muris | - |
- |
- |
| Cryptosporidium muris | B6AJN7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity column chromatography | Cryptosporidium andersoni |
| Ni-affinity column chromatography | Cryptosporidium muris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Cryptosporidium muris | ADP + phosphocreatine | - |
? | |
| ATP + creatine | - |
Cryptosporidium andersoni | ADP + phosphocreatine | - |
? | |
| ATP + creatine | the enzyme has a 20fold greater preference for creatine compared to glycocyamine | Cryptosporidium muris | ADP + phosphocreatine | - |
? | |
| ATP + creatine | the enzyme shows a 20fold greater preference for creatine than glycocyamine | Cryptosporidium muris | ADP + phosphocreatine | - |
? | |
| ATP + glycocyamine | - |
Cryptosporidium muris | ADP + phosphoglycocyamine | - |
? | |
| ATP + glycocyamine | - |
Cryptosporidium andersoni | ADP + phosphoglycocyamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, SDS-PAGE | Cryptosporidium muris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
glycocyamine | at pH 8.5 and 25°C | Cryptosporidium muris | |
| 30 | - |
Creatine | pH and temperature not specified in the publication | Cryptosporidium muris | |
| 30 | - |
Creatine | at pH 8.5 and 25°C | Cryptosporidium muris | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cryptosporidium andersoni | |
| ATP | - |
Cryptosporidium muris | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ATP | in form of Mg-ATP, with creatine as substrate, at pH 8.5 and 25°C | Cryptosporidium muris | |
| 0.3 | - |
ATP | in form of Mg-ATP, with glycocyamine as substrate, at pH 8.5 and 25°C | Cryptosporidium muris | |
| 0.33 | - |
Creatine | at pH 8.5 and 25°C | Cryptosporidium muris | |
| 1.2 | - |
glycocyamine | at pH 8.5 and 25°C | Cryptosporidium muris | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
glycocyamine | at pH 8.5 and 25°C | Cryptosporidium muris | |
| 15.4 | - |
Creatine | pH and temperature not specified in the publication | Cryptosporidium muris | |
| 15.4 | - |
Creatine | at pH 8.5 and 25°C | Cryptosporidium muris | |
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