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Literature summary for 2.7.2.7 extracted from

  • Sirobhushanam, S.; Galva, C.; Saunders, L.P.; Sen, S.; Jayaswal, R.; Wilkinson, B.J.; Gatto, C.
    Utilization of multiple substrates by butyrate kinase from Listeria monocytogenes (2017), Biochim. Biophys. Acta, 1862, 283-290 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Listeria monocytogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6
-
 Isobutyrate at pH 7.5 and 37°C Listeria monocytogenes
13.1
-
 pentanoate at pH 7.5 and 37°C Listeria monocytogenes
16.5
-
 2-ethylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
27.3
-
 3-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
36.2
-
 2-methylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
44.2
-
 2-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
50.5
-
 Butanoate at pH 7.5 and 37°C Listeria monocytogenes
57.1
-
 Isovalerate at pH 7.5 and 37°C Listeria monocytogenes
174.1
-
 propionate at pH 7.5 and 37°C Listeria monocytogenes
1856
-
 hexanoate at pH 7.5 and 37°C Listeria monocytogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 4 mM used in assay conditions Listeria monocytogenes

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
-
-
Listeria monocytogenes 10403S
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-ethylbutyrate
-
 Listeria monocytogenes ADP + 2-ethylbutyryl phosphate
-
 ?
ATP + 2-methylbutyrate
-
 Listeria monocytogenes ADP + 2-methylbutyryl phosphate
-
 ?
ATP + 2-methylpentanoate
-
 Listeria monocytogenes ADP + 2-methylpentanoyl phosphate
-
 ?
ATP + 3-methylpentanoate
-
 Listeria monocytogenes ADP + 3-methylpentanoyl phosphate
-
 ?
ATP + acetate
-
 Listeria monocytogenes ADP + acetyl phosphate
-
 ?
ATP + butanoate third best substrate Listeria monocytogenes ADP + butanoyl phosphate
-
 ?
ATP + hexanoate
-
 Listeria monocytogenes ADP + hexanoyl phosphate
-
 ?
ATP + isobutyrate second best substrate Listeria monocytogenes ADP + isobutyroyl phosphate
-
 ?
ATP + isovalerate
-
 Listeria monocytogenes ADP + isovaleroyl phosphate
-
 ?
ATP + pentanoate best substrate Listeria monocytogenes ADP + pentanoyl phosphate
-
 ?
ATP + propionate
-
 Listeria monocytogenes ADP + propionyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Listeria monocytogenes

Synonyms

Synonyms Comment Organism
Buk
-
 Listeria monocytogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 2-ethylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.27
-
 2-methylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.31
-
 2-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
0.35
-
 3-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
0.4
-
 Isobutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.42
-
 Isovalerate at pH 7.5 and 37°C Listeria monocytogenes
0.84
-
 Butanoate at pH 7.5 and 37°C Listeria monocytogenes
1.2
-
 pentanoate at pH 7.5 and 37°C Listeria monocytogenes
2.59
-
 propionate at pH 7.5 and 37°C Listeria monocytogenes
21.85
-
 hexanoate at pH 7.5 and 37°C Listeria monocytogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.007
-
 Isovalerate at pH 7.5 and 37°C Listeria monocytogenes
0.007
-
 2-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
0.007
-
 2-ethylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.011
-
 hexanoate at pH 7.5 and 37°C Listeria monocytogenes
0.013
-
 3-methylpentanoate at pH 7.5 and 37°C Listeria monocytogenes
0.015
-
 propionate at pH 7.5 and 37°C Listeria monocytogenes
0.017
-
 Butanoate at pH 7.5 and 37°C Listeria monocytogenes
0.052
-
 Isobutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.07
-
 2-methylbutyrate at pH 7.5 and 37°C Listeria monocytogenes
0.092
-
 pentanoate at pH 7.5 and 37°C Listeria monocytogenes