Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-methionine | displays slight activation | Streptococcus mutans |
General Stability | Organism |
---|---|
feedback inhibitor pair protects the enzyme against heat denaturation | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | concerted feedback inhibition with L-threonine; L-threonine methyl ester and L-threonine amide are able to substitute for L-threonine in feedback inhibition, but the requirement for L-lysine is strict | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
ATP | pH 8.1, 29°C | Streptococcus mutans | |
5.5 | - |
L-aspartate | pH 8.1, 29°C | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
6 * 40000, SDS-PAGE | Streptococcus mutans |
242000 | - |
gel filtration | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Streptococcus mutans BHT | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus mutans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.92 | - |
- |
Streptococcus mutans |
Storage Stability | Organism |
---|---|
-20°C, appears to be stable over a period of several months' storage | Streptococcus mutans |
4°C, during 24 h of storage the loss of enzyme activity is substantial | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Streptococcus mutans | ADP + 4-phospho-L-aspartate | - |
? | |
ATP + L-aspartate | - |
Streptococcus mutans BHT | ADP + 4-phospho-L-aspartate | - |
? | |
additional information | strict requirement for ATP as a phosphorylating agent, CTP and GTP are not active | Streptococcus mutans | ? | - |
? | |
additional information | strict requirement for ATP as a phosphorylating agent, CTP and GTP are not active | Streptococcus mutans BHT | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40000, SDS-PAGE | Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 35 | stable in this range | Streptococcus mutans |
50 | - |
about 50% of the enzyme is inactivated after 20 min | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Streptococcus mutans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | in pH regions below 6.0 and above 9.0 the enzyme activity rapidly decreases | Streptococcus mutans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-lysine | pH 8.1, 29°C, noncompetitive inhibition with respect to aspartate, mixed with respect to ATP | Streptococcus mutans |