Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2 DE3 cells | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor Sda, crystals only form in the presence of ADP-Mg2+ (but not with ATP or AMPPNP, and not in the absence of nucleotide) | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sda | potent inhibitor of KinB autophosphorylation, Sda directly inhibits sporulation histidine kinase in response to DNA damage and replication defects, Sda acts to sterically block communication between the catalytic ATP-binding and dimerization/histidinephosphotransfer domains required for autophosphorylation, as well as to sterically block communication between the response regulator Spo0F and DHp domain required for phosphotransfer and phosphatase activities | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation in the presence of ATP | Geobacillus stearothermophilus |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography, followed by anion exchange chromatography and gel filtration | Geobacillus stearothermophilus |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
KinB | - |
Geobacillus stearothermophilus |
sporulation histidine kinase | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |