Literature summary for 2.7.11.8 extracted from

Amin, S.A.; Ghosh, K.; Gayen, S.; Jha, T.
Chemical-informatics approach to COVID-19 drug discovery Monte Carlo based QSAR, virtual screening and molecular docking study of some in-house molecules as papain-like protease (PLpro) inhibitors (2020), J. Biomol. Struct. Dyn., 2020, 1-10 .

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Cloned(Commentary)

Cloned (Comment)	Organism
kinase domain of FASTK353-444 is cloned in pET28a, and the recombinant FASTK is expressed in Escherichia coli BL21 (DE3) cells	Homo sapiens

Organic Solvent Stability

Organic Solvent	Comment	Organism
urea	the folding/unfolding transitions of urea-induced denaturation is monitored with the help of circular dichroism, intrinsic fluorescence, and UV absorption spectroscopies. Analysis of transition curves obtained from different probes reveal a coincidence of denaturation curves, suggesting that folding/unfolding of FASTK follows a two-state process with the midpoint (Cm) value at 3.50 M. Urea-induced denaturation curves are further analyzed to estimate change in the Gibbs free energy in the absence of urea (DGD0) associated with the equilibrium of denaturation. To get atomistic insights into the urea-induced denaturation of FASTK, an all-atom molecular dynamics simulation for 100 ns is performed	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q14296	-	-

Purification (Commentary)

Purification (Comment)	Organism
kinase domain of FASTK353-444	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
the folding/unfolding transitions of urea-induced denaturation is monitored with the help of circular dichroism, intrinsic fluorescence, and UV absorption spectroscopies	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Fas-activated serine/threonine kinase	-	Homo sapiens

General Information

General Information	Comment	Organism
drug target	FASTK is an important protein with its valuable implications in various diseases as it serves as a potential drug target	Homo sapiens
physiological function	the enzyme (FASTK) is a mitochondria-associated nuclear protein that inhibits Fas- and UV-induced apoptosis	Homo sapiens

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Release 2023.1 (January 2023)