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Literature summary for 2.7.11.7 extracted from

  • Russ, M.; Croft, D.; Ali, O.; Martinez, R.; Steimle, P.A.
    Myosin heavy chain kinase A from Dictyostelium possesses a novel actin binding domain that cross-links actin filaments (2005), Biochem. J., 395, 373-383.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
F-actin i.e. filamentous actin, from rabbit muscle, about 40fold activation, coiled-coil domain binding structure and mechanism, overview, the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged isozyme MHCK A in a Dictyostelium discoideum strain lacking endogenous MHCK A, expression of a truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Escherichia coli as GST-fused protein Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information the truncation mutant DELTAcoil-MHCK A comprising residues 1-498 is not affected in its autophosphorylation rate by F-actin Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information F-actin binding kinetics Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A Dictyostelium discoideum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin-heavy-chain] Dictyostelium discoideum
-
 ADP + [myosin-heavy-chain]phosphate
-
 ?
additional information Dictyostelium discoideum C-terminal phosphorylation of myosin heavy chain is required for regulation of myosin II filament assembly, regulation mechanism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P42527 isozyme MHCK A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length isozyme MHCK A from Dictyostelium discoideum by affinity chromatography, recombinant GST-fusion truncation mutant DELTAcoil-MHCK A from Escherichia coli by affinity chromatography, the GST-tag is cleaved off by thrombin treatment Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin-heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin-heavy-chain]phosphate
-
 ?
additional information C-terminal phosphorylation of myosin heavy chain is required for regulation of myosin II filament assembly, regulation mechanism Dictyostelium discoideum ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A Dictyostelium discoideum

Synonyms

Synonyms Comment Organism
MHCK A
-
 Dictyostelium discoideum
myosin heavy chain kinase A
-
 Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 actin binding assay at Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 actin binding assay at Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum