Cloned (Comment) | Organism |
---|---|
expression of the catalytic domain of MHCK B as a GST-fusion protein in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
expression of the His-tagged catalytic domain of MHCK A in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
expression of the His-tagged catalytic domain of MHCK C in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
0.55 | - |
YAYDTRYRR | pH 7.0, 25°C | Dictyostelium discoideum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin II heavy chain | Dictyostelium discoideum | - |
ADP + myosin II heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | O76739 | gene MHCK C, myosin II heavy chain kinase C | - |
Dictyostelium discoideum | P42527 | gene MHCK A, myosin II heavy chain kinase A | - |
Dictyostelium discoideum | P90648 | gene MHCK B, myosin II heavy chain kinase B | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion catalytic domain of MHCK B, expressed in Escherichia coli | Dictyostelium discoideum |
recombinant His-tagged catalytic domain of MHCK A, expressed in Escherichia coli | Dictyostelium discoideum |
recombinant His-tagged catalytic domain of MHCK C, expressed in Escherichia coli | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + caldesmon | catalytic domain | Dictyostelium discoideum | ADP + caldesmon phosphate | - |
? | |
ATP + casein | catalytic domain, low activity | Dictyostelium discoideum | ADP + casein phosphate | - |
? | |
ATP + myelin basic protein | catalytic domain | Dictyostelium discoideum | ADP + myelin basic protein phosphate | - |
? | |
ATP + myosin II heavy chain | - |
Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | catalytic domain | Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | phosphorylates primarly threonine residues | Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + myosin regulatory light chain | catalytic domain | Dictyostelium discoideum | ADP + myosin regulatory light chain phosphate | - |
? | |
ATP + RKKFGESEKTKTKEFL-amide | low activity | Dictyostelium discoideum | ? | - |
? | |
ATP + RKKFGESEKTKTKEFL-amide | catalytic domain | Dictyostelium discoideum | ? | - |
? | |
ATP + RKKFGESEKTKTKEFL-amide | synthetic peptide MH-3 | Dictyostelium discoideum | ? | - |
? | |
ATP + YAYDTRYRR | consensus sequence | Dictyostelium discoideum | ? | - |
? | |
ATP + YAYDTRYRR | catalytic domain of the enzyme | Dictyostelium discoideum | ? | - |
? | |
additional information | localization of autophosphorylation sites | Dictyostelium discoideum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MHCK | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dictyostelium discoideum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Dictyostelium discoideum | |
additional information | - |
additional information | catalytic domain, several substrates | Dictyostelium discoideum | |
0.01 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
0.55 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
0.79 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
14 | - |
YAYDTRYRR | pH 7.0, 25°C | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |