Literature summary for 2.7.11.5 extracted from

Oudot, C.; Cortay, J.C.; Blanchet, C.; Laporte, D.C.; Di Pietro, A.; Cozzone, A.J.; Jault, J.M.
The "catalytic" triad of isocitrate dehydrogenase kinase/phosphatase from E. coli and its relationship with that found in eukaryotic protein kinases (2001), Biochemistry, 40, 3047-3055.

Search for more literature for 2.7.11.5

Protein Variants

Protein Variants	Comment	Organism
D403A	site-directed mutagenesis	Escherichia coli
E439A	site-directed mutagenesis	Escherichia coli
N377A	site-directed mutagenesis	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0069	-	ATP	pH 7.5, 37°C, wild-type	Escherichia coli
0.0087	-	ATP	pH 7.5, 37°C, mutant D403A	Escherichia coli
0.0098	-	ATP	pH 7.5, 37°C, mutant Glu439Ala	Escherichia coli
0.0147	-	ATP	pH 7.5, 37°C, mutant Asn377Ala	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	2 * 66000, homodimer, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [isocitrate dehydrogenase (NADP+)]	Escherichia coli	-	ADP + [isocitrate dehydrogenase (NADP+)] phosphate	-	r
ATP + [isocitrate dehydrogenase (NADP+)]	Escherichia coli JM109	-	ADP + [isocitrate dehydrogenase (NADP+)] phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P11071	JM109	-
Escherichia coli JM109	P11071	JM109	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [isocitrate dehydrogenase (NADP+)]	-	Escherichia coli	ADP + [isocitrate dehydrogenase (NADP+)] phosphate	-	r
ATP + [isocitrate dehydrogenase (NADP+)]	-	Escherichia coli JM109	ADP + [isocitrate dehydrogenase (NADP+)] phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 66000, homodimer, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
IDHK/P	-	Escherichia coli
isocitrate dehydrogenase kinase/phosphatase	-	Escherichia coli

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Release 2023.1 (January 2023)