Cloned (Comment) | Organism |
---|---|
expression of His6-tagged MEKK1 subdomain VIII, comprising residues 1174-1493, His6-tagged wild-type, full-length enzyme, and His6-tagged mutant enzymes F1443A, I1394/L1402A, Q1405R/Q1406R, and L1402A/F1443A in HeLa and COS-1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F1443A | site-directed mutagenesis, subdomain X mutation, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
I1394/L1402A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
L1402A/F1443A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
Q1405R/Q1406R | site-directed mutagenesis C-terminal to the subdomain VIII, creation of an optimal recognition site for protease furin, the mutant binds MKK4 more tightly than the wild-type enzyme, MKK4 binding protects the mutant enzyme from proteolytic cleavage | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK4 | Mus musculus | MKK4 activates the JNK signaling pathway | ADP + phosphorylated MKK4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK4 | MKK4 activates the JNK signaling pathway | Mus musculus | ADP + phosphorylated MKK4 | - |
? | |
ATP + MKK4 | a MAPKK, activity of wild-type and mutant MEKK1 with wild-type and mutant MKK4, determination of the MKK4 binding site of MEKK1, overview | Mus musculus | ADP + phosphorylated MKK4 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | subdomain VIII is a specificity-determining region in MEKK1 | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
MEKK1 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |