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Literature summary for 2.7.11.24 extracted from
- Evidence for a specific and critical role of mitogen-activated protein kinase 20 in uni-to-binucleate transition of microgametogenesis in tomato (2018), New Phytol., 219, 176-194 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Solanum lycopersicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Solanum lycopersicum | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MYB32 | Solanum lycopersicum | - |
ADP + phosphorylated MYB32 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
cultivar Micro-Tom | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel column chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| carpel | - |
Solanum lycopersicum | - |
| microspore | uninuclear microspores | Solanum lycopersicum | - |
| additional information | not detected in microspore mother cells or mature pollen grains | Solanum lycopersicum | - |
| petal | - |
Solanum lycopersicum | - |
| pollen | binuclear pollen | Solanum lycopersicum | - |
| sepal | - |
Solanum lycopersicum | - |
| stamen | highest expression | Solanum lycopersicum | - |
| tetrad of microspores | - |
Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MYB32 | - |
Solanum lycopersicum | ADP + phosphorylated MYB32 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Solanum lycopersicum |
| ? | x * 70600, calculated from amino acid sequence | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mitogen-activated protein kinase 20 | - |
Solanum lycopersicum |
| MPK20 | - |
Solanum lycopersicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | knockout of mitogen-activated protein kinase 20 significantly reduces the expression of a large number of genes controlling sugar and auxin metabolism and signaling in anthers. Suppression or knockout of the enzyme expression leads to abnormal pollen development | Solanum lycopersicum |
| physiological function | mitogen-activated protein kinase 20 exerts its role specifically at the uni-to-binucleate transition during microgametogenesis. The enzyme specifically regulates post-meiotic pollen development through modulating sugar and auxin metabolism and signaling | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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