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Literature summary for 2.7.11.23 extracted from

  • Allepuz-Fuster, P.; Martinez-Fernandez, V.; Garrido-Godino, A.I.; Alonso-Aguado, S.; Hanes, S.D.; Navarro, F.; Calvo, O.
    Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation (2014), Nucleic Acids Res., 42, 13674-13688.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.23

Activating Compound

Activating Compound Comment Organism Structure
additional information the Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAPII) facilitate RNA polymerase II CTD dephosphorylation Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
T162A the kin28-T162A allele, which does not display a slow growth phenotype, has reduced activity in vitro, but does not detectably affect CTD phosphorylation and capping enzymes recruitment in vivo Saccharomyces cerevisiae
T17D the mutant has decreased CTD kinase activity, a defect in capping enzyme recruitment, and is slow growing at 25°C and 37°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
 Saccharomyces cerevisiae 785
-
nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [DNA-directed RNA polymerase] Saccharomyces cerevisiae CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
ATP + [DNA-directed RNA polymerase] Saccharomyces cerevisiae KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
ATP + [DNA-directed RNA polymerase] Saccharomyces cerevisiae SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 ADP + phospho-[DNA-directed RNA polymerase]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P06242
-
-
Saccharomyces cerevisiae P39073
-
-
Saccharomyces cerevisiae Q03957
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [DNA-directed RNA polymerase] CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
ATP + [DNA-directed RNA polymerase] KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
ATP + [DNA-directed RNA polymerase] SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5 Saccharomyces cerevisiae ADP + phospho-[DNA-directed RNA polymerase]
-
 ?

Synonyms

Synonyms Comment Organism
CTD kinase
-
 Saccharomyces cerevisiae
Ctk1
-
 Saccharomyces cerevisiae
Kin28
-
 Saccharomyces cerevisiae
Ser5/Ser7 kinase
-
 Saccharomyces cerevisiae
Srb10
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism phosphorylation state of the C-terminal domain of RNA polymerase II, detailed overview Saccharomyces cerevisiae
physiological function Ctk1, the main Ser2 kinase in coding and 3'-end regions, is required for cotranscriptional recruitment of the polyadenylation machinery. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants Saccharomyces cerevisiae
physiological function Kin28 (mammalian Cdk7), a Ser5/Ser7 kinase, facilitates transcription and formation of the scaffold complex together with Ser2/Ser5 kinase Srb10 (mammalian Cdk8). Kin28 is part of the TFIIH initiation complex and phosphorylates Ser5 to promote cotranscriptional 5' mRNA capping, and in mammals also phosphorylates Ser7 during promoter-proximal pausing and perhaps during termination. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants Saccharomyces cerevisiae
physiological function Srb10 (mammalian Cdk8), a Ser2/Ser5 kinase, is part of the Mediator complex and inactivatesRNAPII prior to pre-initiation complex formation, and, together with Kin28 (a Ser5/Ser7 kinase), facilitates transcription and formation of the scaffold complex. The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II as Rpb4/7 heterodimer is important to maintain proper levels of RNAPII phosphorylation, and it functions by a mechanism(s) that involves Rpb4/7-dependent association, recruitment and/or accessibility, of key CTD modifying enzymes, phosphorylation of RNAPII CTD is altered in Rpb4/7 mutants Saccharomyces cerevisiae