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Literature summary for 2.7.11.23 extracted from

  • Badjatia, N.; Ambrosio, D.L.; Lee, J.H.; Guenzl, A.
    Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of the RNA polymerase II subunit RPB1 (2013), Mol. Cell. Biol., 33, 1965-1975.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene CRK9, quantitative real-time reverse transcription-PCR expression analysis Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information CRK9 silencing by expression of RNAi Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [DNA-directed RNA polymerase] Trypanosoma brucei enzyme phosphorylates residues in the C-terminal domain, CTD, of the largest RNA polymerase II (RNA Pol II) subunit, RPB1 ADP + phospho-[DNA-directed RNA polymerase]
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 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [DNA-directed RNA polymerase] enzyme phosphorylates residues in the C-terminal domain, CTD, of the largest RNA polymerase II (RNA Pol II) subunit, RPB1 Trypanosoma brucei ADP + phospho-[DNA-directed RNA polymerase]
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 ?

Synonyms

Synonyms Comment Organism
cdc2-related kinase 9
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 Trypanosoma brucei
CRK9
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 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma brucei

General Information

General Information Comment Organism
evolution protistan organisms, such as amoebozoa, kinetoplastids, trichomonads, and diplomonads, which have diverged very early from other eukaryotic lineages, lack repetitive CTD motifs Trypanosoma brucei
malfunction silencing the expression of Trypanosoma brucei cdc2-related kinase 9 (CRK9) leads to a loss of RPB1 phosphorylation, but that does not impair RNA Pol II transcription or cotranscriptional m7G capping. Instead, CRK9 silencing leads to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA maturation, that is caused by hypomethylation of the SL RNA's unique cap4. Trypanosoma brucei
physiological function human parasite Trypanosoma brucei lacks the heptad repeats and a CDK7 orthologue. Characterization of trypanosome TFIIH does not identify a kinase component. The Trypanosoma brucei CTD nevertheless is phosphorylated by cdc2-related kinase 9 (CRK9) and is essential for transcription. RPB1 phosphorylation and SL RNA cap4 formation depend on CRK9 kinase activity. CRK9-dependent RPB1 phosphorylation is neither required for transcription initiation at the SLRNA promoter nor an essential modification for transcription elongation within the protein coding gene arrays Trypanosoma brucei