Application | Comment | Organism |
---|---|---|
additional information | role for PLK kinase activity in basal body and kinetoplast migration, PLK is essential for cytokinesis furrow ingression in bloodstream-form Trypanosoma brucei | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
overexpression of TY1-epitope-tagged PLK and a kinase-dead variant, results in the accumulation of cells that have divided their nucleus but not their kinetoplast (2N1K cells), kinetoplast is mainly found at the posterior end of the cell | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
N169A | abrogates kinase activity, after PLK depletion the single kinetoplast is predominantly located between the two divided nuclei | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA | downregulates PLK, in procyclic forms inhibition of kinetoplast division, in bloodstream form also delayed kinetoplast division, which is further observed to inhibit furrow ingression during cytokinesis | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Trypanosoma brucei | 5737 | - |
additional information | no localization to the nucleus, kinetoplast, basal bodies or to the flagellum | Trypanosoma brucei | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | - |
Trypanosoma brucei | - |
procyclic form | - |
Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-casein | - |
Trypanosoma brucei | ADP + phosphorylated alpha-casein | - |
? | |
ATP + beta-casein | - |
Trypanosoma brucei | ADP + phosphorylated beta-casein | - |
? | |
additional information | no activity against myelin basic protein or histone H1 | Trypanosoma brucei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLK | - |
Trypanosoma brucei |
polo-like kinase | - |
Trypanosoma brucei |