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Literature summary for 2.7.11.21 extracted from

  • Elowe, S.; Huemmer, S.; Uldschmid, A.; Li, X.; Nigg, E.A.
    Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions (2007), Genes Dev., 21, 2205-2219.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Application

Application Comment Organism
additional information Plk1 is responsible for causing mitotic BubR1 upshift, and attribute a kinetochore-specific function to the hyperphosphorylated form of BubR1 in the stabilization of kinetochore-microtubule interactions Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information polo-box domain mutant in which residues His 538 and Lys 540, critical for ligand binding, are changed to alanine, do not pull down BubR1 from mitotic cell lysates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
siRNA depletion of Plk1, which leads to loss of BubR1 hyperphosphorylation, but has no significant effects on BubR1 localization Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetochore kinetochore localization of BubR1 and Plk1 is important for BubR1 hyperphosphorylation Homo sapiens 776
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + BubR1 Homo sapiens S676 is an in vivo Plk1 phosphorylation site in BubR1 ADP + phosphorylated BubR1
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53350
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Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + BubR1 S676 is an in vivo Plk1 phosphorylation site in BubR1 Homo sapiens ADP + phosphorylated BubR1
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 ?
ATP + BubR1 Plk1-BubR1 interaction is mediated by polo-box domain binding to pT620 of BubR1. S676 is a Plk1-specific phosphorylation site, site is phosphorylated during prometaphase, but dephosphorylated at metaphase upon establishment of tension between sister chromatids. Phosphorylation is not required for spindle checkpoint function but instead is important for the stability of kinetochore-microtubule interactions, timely mitotic progression, and chromosome alignment onto the metaphase plate Homo sapiens ADP + phosphorylated BubR1
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 ?

Synonyms

Synonyms Comment Organism
Plk1
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 Homo sapiens
Polo-like kinase 1
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 Homo sapiens