Cloned (Comment) | Organism |
---|---|
expression of HA-tagged Plk in HeLa cells, co-expression of His-tagged Plk with wild-type and mutant Myt1 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Myt1 | Homo sapiens | phosphorylation of Myt1 during M phase | ADP + phosphorylated Myt1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant HA-tagged Plk from HeLa cells by immunoprecipitation | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | the Plk consensus sequence is D/E-X-S/T-hydrophobic amino acid-X-D/E with X being any amino acid | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Myt1 | phosphorylation of Myt1 during M phase | Homo sapiens | ADP + phosphorylated Myt1 | - |
? | |
ATP + Myt1 | i.e. membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, no activity with a Myt1 mutant in which the 4 C-terminal phosphorylation sites are mutated to alanine | Homo sapiens | ADP + phosphorylated Myt1 | - |
? | |
additional information | determination of enzyme consensus sequence | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLK | - |
Homo sapiens |
polo-like kinase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |