Cloned (Comment) | Organism |
---|---|
cooverexpression with the beta subunit of the rabbit enzyme with the rat holoenzyme and rat alphagammadelta and gammadelta subunit complexes in Spodoptera frugiperda Sf9 cells via the baculovirus infection system, resulting in formation of subunit subcomplexes, overview | Oryctolagus cuniculus |
subcloning of subunits in Escherichia coli, overexpression of the soluble holoenzyme and of soluble trimeric alphagammadelta and dimeric gammadelta subunit subcomplexes, as well as coexpression with the beta subunit of the rabbit enzyme also resulting in subunit complex formation, in Spodoptera frugiperda Sf9 cells via the baculovirus infection system | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, dependent on, required for binding of calmodulin | Oryctolagus cuniculus | |
Ca2+ | activates, dependent on, required for binding of calmodulin | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | Q64649 | alpha subunit of the skeletal muscle enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the native and the recombinant enzyme performs autophosphorylation of its alpha and beta subunits | Oryctolagus cuniculus |
phosphoprotein | regulation by de-/phosphorylation performed by cAMP-dependent protein kinase, EC 2.7.11.11 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
native enzyme from skeletal muscle, recombinant enzyme subunit subcomplexes from Sf9 insect cells | Oryctolagus cuniculus |
recombinant holoenzyme and enzyme subunit subcomplexes from Sf9 insect cells | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of purified native and recombinant enzymes and subunit subcomplexes at different pH | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme performs autophosphorylation | Oryctolagus cuniculus | ? | - |
? | |
additional information | the enzyme performs autophosphorylation | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexadecamer | (alphabetagammadelta)4 | Rattus norvegicus |
More | the delta subunit is identical with calmodulin | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PhK | - |
Oryctolagus cuniculus |
PhK | - |
Rattus norvegicus |
Phosphorylase b kinase | - |
Oryctolagus cuniculus |
Phosphorylase b kinase | - |
Rattus norvegicus |
SkM Phk | - |
Oryctolagus cuniculus |
SkM Phk | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
autophosphorylation reaction at | Oryctolagus cuniculus |
30 | - |
autophosphorylation reaction at | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity of purified native and recombinant enzymes and subunit subcomplexes at different pH, the latter show higher activity at pH 6.8, while the native enzyme shows higher activity at pH 8.2 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus | |
ATP | - |
Rattus norvegicus | |
Calmodulin | Ca2+-dependent binding, identical with the delta subunit | Oryctolagus cuniculus | |
Calmodulin | Ca2+-dependent binding, identical with the delta subunit | Rattus norvegicus |